• Publications
  • Influence
Two discriminatory binding sites in the Escherichia coli replication origin are required for DNA strand opening by initiator DnaA-ATP.
Initiation of DNA replication in eukaryotes, archea, and eubacteria requires interaction of structurally conserved ATP-binding initiator proteins and origin DNA to mediate assembly of replisomes.Expand
Metabolites from an Antarctic sponge-associated bacterium, Pseudomonas aeruginosa.
In an ongoing survey of the bioactive potential of microorganisms associated with marine invertebrates, the culture media of a sponge-associated bacterial strain of Pseudomonas aeruginosa was foundExpand
Correlation of gene transcription with the time of initiation of chromosome replication in Escherichia coli
Transcriptional levels of the Escherichia coli mioC and gidA genes, which flank the chromosomal origin of replication (oriC) and the dnaA gene, were correlated with the time of initiation ofExpand
DNA replication origins.
The onset of genomic DNA synthesis requires precise interactions of specialized initiator proteins with DNA at sites where the replication machinery can be loaded. These sites, defined as replicationExpand
Regulation of DnaA assembly and activity: taking directions from the genome.
To ensure proper timing of chromosome duplication during the cell cycle, bacteria must carefully regulate the activity of initiator protein DnaA and its interactions with the unique replicationExpand
SeqA blocking of DnaA-oriC interactions ensures staged assembly of the E. coli pre-RC.
DnaA occupies only the three highest-affinity binding sites in E. coli oriC throughout most of the cell cycle. Immediately prior to initiation of chromosome replication, DnaA interacts withExpand
IHF and HU stimulate assembly of pre‐replication complexes at Escherichia coli oriC by two different mechanisms
Pre‐replication complexes (pre‐RC) assemble on replication origins and unwind DNA in the presence of chromatin proteins. As components of Escherichia coli pre‐RC, two histone‐like proteins HU and IHFExpand
The orisome: structure and function
During the cell division cycle of all bacteria, DNA-protein complexes termed orisomes trigger the onset of chromosome duplication. Orisome assembly is both staged and stringently regulated to ensureExpand
Two oppositely oriented arrays of low‐affinity recognition sites in oriC guide progressive binding of DnaA during Escherichia coli pre‐RC assembly
The onset of chromosomal DNA replication requires highly precise and reproducible interactions between initiator proteins and replication origins to assemble a pre‐replicative complex (pre‐RC) thatExpand
Regulating DnaA complex assembly: it is time to fill the gaps.
New rounds of bacterial chromosome replication are triggered during each cell division cycle by the initiator protein, DnaA. For precise timing, interactions of DnaA-ATP monomers with the replicationExpand