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PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples
These PCR assays can rapidly define the occurrence, species incidence, and flaA genotypes of enteropathogenic campylobacters.
Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay.
A PCR-based study of the incidence of enteropathogenic campylobacter infection in humans was done on the basis of a detection and identification algorithm consisting of screening PCRs and species identification by PCR-enzyme-linked immunosorbent assay, and there was no statistically significant difference between PCR and culture in detection of C. jejuni-C.
A national outbreak of multi-resistant Salmonella enterica serovar Typhimurium definitive phage type (DT) 104 associated with consumption of lettuce
The complexity of the food supply chain and the lack of identifying markers on salad stuffs made tracking salad vegetables back to their origin extremely difficult in most instances.
Campylobacter lanienae sp. nov., a new species isolated from workers in an abattoir.
- J. Logan, A. Burnens, D. Linton, A. Lawson, J. Stanley
- Biology, MedicineInternational journal of systematic and…
- 1 March 2000
Nucleotide sequence analysis of the 16S rRNA gene, DNA homology experiments and determination of G + C content demonstrated that these strains constituted a previously undescribed species, whose nearest phylogenetic neighbours were Campylobacter hyointestinalis subsp.
Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods
- S. Kulkarni, S. Lever, J. Logan, A. Lawson, J. Stanley, M. Shafi
- Biology, MedicineJournal of clinical pathology
- 1 October 2002
Selective culture is as good as the PCR identification algorithm for the detection of the two most common species, C jejuni and C coli, and it is cheap and practical, but it does miss the less common species and results take 48 hours, and identification is only to the genus level.
Subtyping intimin genes from enteropathogenic Escherichia coli associated with outbreaks and sporadic cases in the United Kingdom and Eire.
Polymerase chain reaction detection and speciation of Campylobacter upsaliensis and C. helveticus in human faeces and comparison with culture techniques
- A. Lawson, D. Linton, J. Stanley, R. Owen
- Biology, MedicineJournal of applied microbiology
- 1 August 1997
A polymerase chain reaction (PCR) assay based on the 16S rRNA gene and an improved DNA extraction procedure were developed for the direct detection and differentiation of Campylobacter upsaliensis and C. helveticus in seeded human faeces and accurately identified the two phenotypically similar species.
Campylobacter hominis sp. nov., from the human gastrointestinal tract.
The unique species status of the isolates was further confirmed by taxonomic analysis of 47 phenotypic characteristics, and the 16S rDNA nucleotide sequence analysis were consistent with a new Campylobacter species whose nearest phylogenetic neighbours were Campylabacter gracilis and CampylOBacter sputorum.
Use of a LightCycler gyrA Mutation Assay for Rapid Identification of Mutations Conferring Decreased Susceptibility to Ciprofloxacin in Multiresistant Salmonella enterica Serotype Typhimurium DT104…
This study confirmed that MR DT104 with decreased susceptibility to ciprofloxacin from humans and food animals in England and Wales may have arisen independently against a background of clonal spread of MRDT104.
Detection of Oxazolidinone-Resistant Enterococcus faecalis and Enterococcus faecium Strains by Real-Time PCR and PCR-Restriction Fragment Length Polymorphism Analysis
A real-time PCR assay identified linezolid-resistant Enterococcus faecalis and Enterococces faecium isolates with a G2576U rRNA mutation that may aid surveillance and individual case management.