Synthesis of a variety of optically active hydroxylated heterocyclic compounds using epoxide hydrolase technology
- Daniel P Pienaar, R. Mitra, T. V. Deventer, A. L. Botes
- Chemistry
- 24 November 2008
Biocatalytic resolution of 1,2-epoxyoctane using resting cells of different yeast strains with novel epoxide hydrolase activities
- A. L. Botes, C. Weijers, M. V. van Dyk
- Biology, EngineeringBiotechnology Letters
- 1 April 1998
Good enantioselectivity (E > 200) for 1,2-epoxyoctane is reported for the first time and asymmetric hydrolysis for 8 yeast strains belonging to the genera Trichosporon, Rhodotorula, and Rhodosporidium is found.
Enantioselective Hydrolysis of Unbranched Aliphatic 1,2-Epoxides by Rhodotorula glutinis.
- C. Weijers, A. L. Botes, M. V. Dyk, J. Bont
- Chemistry
- 14 July 1998
Enantioselectivities of yeast epoxide hydrolases for 1,2-epoxides
- A. L. Botes, C. Weijers, P. Botes, M. V. Dyk
- Chemistry, Biology
- 27 August 1999
Affinity purification and characterization of a yeast epoxide hydrolase
- A. L. Botes
- BiologyBiotechnology Letters
- 1 June 1999
The first report of a yeast epoxide hydrolase purified to homogeneity in milligram amounts is reported, and the amino acid composition of the protein was determined.
Screening of yeast species for the stereo-selective reduction of bicyclo[2.2.2]octane-2,6-dione
- A. L. Botes, Daniel Harvig, M. Gorwa-Grauslund
- Chemistry, Biology
- 9 April 2002
Yeast strains from more than 31 different genera were screened for the enantioselective reduction of bicyclo-2,6-dione andCandida tropicalis and Candida wickerhamii UOFS Y-0652 displayed this unusual diastereoselectivity.
Cloning and Sequencing of an Epoxide Hydrolase Gene from Rhodosporidium paludigenum
- M. Labuschagné, A. L. Botes, J. Albertyn
- Biology, EngineeringDNA Sequence
- 1 January 2004
The EH encoding gene from Rhodosporidium paludigenum was isolated, cloned and sequenced, and a relative high degree of sequence homology was revealed compared to the amino acid sequence of the EH from Rhodotorula glutinis.
Enantioselective hydrolysis of unbranched aliphatic 1,2-epoxides by Rhodotorula glutinis
- C. Weijers, A. L. Botes, M. V. Dyk, J. Bont
- Chemistry
- 13 February 1998
Physico-chemical properties of the epoxide hydrolase from Rhodosporidium toruloides
- A. L. Botes, D. Litthauer, A. van Tonder, M. V. van Dyk
- Biology, ChemistryBiotechnology Letters
- 1 December 1999
It is proposed that the microsomal epoxide hydrolase from Rhodosporidium toruloides UOFS Y-0471 acts at an interface, analogous to lipases, suggesting that a Ser in the catalytic site is indispensable for substrate binding by analogy of the role of Ser residues in the related L-2-haloacid dehalogenases, as well as the ATPase and phosphatase enzymes.
Interspecies differences in the enantioselectivity of epoxide hydrolases in Cryptococcus laurentii (Kufferath) C.E. Skinner and Cryptococcus podzolicus (Bab'jeva & Reshetova) Golubev.
- A. L. Botes, J. Lotter, O. Rhode, A. Botha
- Biology, ChemistrySystematic and Applied Microbiology
- 28 January 2005
...
...