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New ways to break an old bond: the bacterial carbon-phosphorus hydrolases and their role in biogeochemical phosphorus cycling.
Whole-genome and metagenome sequence analysis indicates that the genes encoding these newly described C-P hydrolases are distributed widely among prokaryotes, and may play a hitherto-unrecognized role in phosphonate breakdown in the environment and hence make a significant contribution to global biogeochemical P-cycling.
The plasmid-located haloalkane dehalogenase gene from Rhodococcus rhodochrous NCIMB 13064.
It was found that reversible rearrangements of DNA in the dhaA region may be responsible for the control of expression of haloalkane dehalogenase in R. rhodochrous NCIMB 13064.
Cloning of new Rhodococcus extradiol dioxygenase genes and study of their distribution in different Rhodococcus strains.
Analysis of six Rhodococcus strains revealed a wide distribution of the above dioxygenase genes, and nucleotide sequences homologous to the edoB gene were present in all of the strains, including R. erythropolis NCIMB 13065, which did not utilize any of the aromatic compounds analysed.
Direct quantification of inorganic polyphosphate in microbial cells using 4'-6-diamidino-2-phenylindole (DAPI).
A direct fluorescence based DAPI assay system which removes the requirement for prior polyP extraction before quantification, which increased the efficiency of polyP detection by 28-55% in microbial cultures suggesting quantitative measurement of the intracellular polyP pool.
Structure and kinetics of phosphonopyruvate hydrolase from Variovorax sp. Pal2: new insight into the divergence of catalysis within the PEP mutase/isocitrate lyase superfamily.
The proposed PPH catalytic mechanism is analogous to that of PEPM but includes activation of a water nucleophile with the loop Thr118 residue and may have favored the open conformation of PPH even when the enzyme was cocrystallized with the oxalate inhibitor.
Structural and Functional Analysis of the Phosphonoacetate Hydrolase (phnA) Gene Region in Pseudomonas fluorescens 23F
- A. Kulakova, L. Kulakov, Natalya V. Akulenko, V. Ksenzenko, J. Hamilton, John P. Quinn
- BiologyJournal of bacteriology
- 1 June 2001
Analysis of the regions adjacent to the phosphonoacetate hydrolase structural gene indicated the presence of five open reading frames (ORFs), including one (phnR) whose putative product shows high levels of homology to the LysR family of positive transcriptional regulators.
A comparison of three bacterial phosphonoacetate hydrolases from different environmental sources
Phosphonoacetate hydrolase activity is now reported in extracts of environmental Curtobacterium sp.
Cloning of the phosphonoacetate hydrolase gene from Pseudomonas fluorescens 23F encoding a new type of carbon-phosphorus bond cleaving enzyme and its expression in Escherichia coli and Pseudomonas…
Plasmid pRTL1 controlling 1-chloroalkane degradation by Rhodococcus rhodochrous NCIMB13064.
High-frequency transfer of the 1-chloroalkane degradation marker associated with pRTL1 has been demonstrated in bacterial crosses between different derivatives of R. rhodochrous NCIMB13064.
Cryptic plasmid pKA22 isolated from the naphthalene degrading derivative of Rhodococcus rhodochrous NCIMB13064.
Two sets of long direct repeats were found in pKA22 which may be involved in the replication of the plasmid and recombination processes.