A. Kopylov

Publications125
h-index15
Citations742
Highly Influential Citations8
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Isatin‐binding proteins of rat and mouse brain: Proteomic identification and optical biosensor validation
Isatin (indole‐2,3‐dione) is an endogenous indole that has a distinct and discontinuous distribution in the brain and in other mammalian tissues and body fluids. Its output is increased under… Expand
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Effect of affinity Sorbent on proteomic profiling of isatin-binding proteins of mouse brain
Use of small molecules for isolation of particular sub-proteomes is often complicated by the need for chemical modification of a parent compound for affinity sorbent preparation. Isatin… Expand
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The Size of the Human Proteome: The Width and Depth
This work discusses bioinformatics and experimental approaches to explore the human proteome, a constellation of proteins expressed in different tissues and organs. As the human proteome is not a… Expand
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Proteomics of mouse liver microsomes: Performance of different protein separation workflows for LC‐MS/MS
The mouse liver microsome proteome was investigated using ion trap MS combined with three separation workflows including SDS‐PAGE followed by reverse‐phase LC of in‐gel protein digestions (519… Expand
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Semi-supervised quality control method for proteome analyses based on tandem mass spectrometry
Abstract With the increasing volume of bottom-up proteome analyses using liquid chromatography/mass spectrometry (LC–MS), a rapid and easy for interpretation assessment of the experimental system… Expand
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Affinity Isolation and Mass Spectrometry Identification of Prostacyclin Synthase (PTGIS) Subinteractome
Prostacyclin synthase (PTGIS; EC 5.3.99.4) catalyzes isomerization of prostaglandin H2 to prostacyclin, a potent vasodilator and inhibitor of platelet aggregation. At present, limited data exist on… Expand
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The Effects of Endogenous Non-Peptide Molecule Isatin and Hydrogen Peroxide on Proteomic Profiling of Rat Brain Amyloid-β Binding Proteins: Relevance to Alzheimer’s Disease?
The amyloid-β peptide is considered as a key player in the development and progression of Alzheimer’s disease (AD). Although good evidence exists that amyloid-β accumulates inside cells,… Expand
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Isatin binding proteins in rat brain: In situ imaging, quantitative characterization of specific [3H]isatin binding, and proteomic profiling
Isatin (indole‐2,3‐dione) is an endogenous indole that has a distinct and discontinuous distribution in the brain and in other mammalian tissues and body fluids. Its output is increased under… Expand
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Chromosome 18 transcriptome profiling and targeted proteome mapping in depleted plasma, liver tissue and HepG2 cells.
The final goal of the Russian part of the Chromosome-centric Human Proteome Project (C-HPP) was established as the analysis of the chromosome 18 (Chr 18) protein complement in plasma, liver tissue… Expand
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Affinity‐based proteomic profiling: Problems and achievements
Affinity chromatography becomes a more and more popular method used in proteomic studies for separation of various groups of proteins (subproteomes). The review highlights the role of affinity… Expand
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