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Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology
A novel method based on strain-dependent hybridization patterns of in vitro-amplified DNA with multiple spacer oligonucleotides was found to differentiate M. bovis from M. tuberculosis, a distinction which is often difficult to make by traditional methods.
Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to (myco)bacteria.
IL-23 rather than IL-12 is the primary type 1 cytokine produced by activated proinflammatory Mphi-1, indicating that Mphi heterogeneity thus may be an important determinant of immunity and disease outcome in intracellular bacterial infection.
A simple and economical direct agglutination test for serodiagnosis and sero-epidemiological studies of visceral leishmaniasis.
A simple and economical direct agglutination test for the detection of visceral leishmaniasis is described and could be used in district hospitals and health centres in endemic areas as an aid in diagnosis of kala-azar and in the field for sero-epidemiological studies.
Improvement of a direct agglutination test for field studies of visceral leishmaniasis
The test could be performed on samples of whole blood; thus the difficulties of preparation and storage of serum, plasma, or filter paper blood are avoided and a single serum dilution of 1:6,400 could be employed, contributing to a further reduction in test expenses.
Application of a direct agglutination test for detection of specific anti-Leishmania antibodies in the canine reservoir
The results of this study demonstrate that the DAT is highly suitable for wide-scale epidemiological and ecological field work and could also facilitate diagnosis of leishmaniasis in dogs in veterinary health services.
A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples
Diagnostic techniques based on PCR have two major problems: false-positive reactions due to contamination with DNA fragments from previous PCRs (amplicons) and false-negative reactions caused by
The 14,000-molecular-weight antigen of Mycobacterium tuberculosis is related to the alpha-crystallin family of low-molecular-weight heat shock proteins
Eight monoclonal antibodies directed against the 14,000-molecular-weight (14K) antigen of Mycobacterium tuberculosis reacted specifically with mycobacteria of the M. tuberculosis complex, recognizing at least four distinct epitopes localized within the following three regions of the 14K protein.
PCR assay based on DNA coding for 16S rRNA for detection and identification of mycobacteria in clinical samples
The results of the PCR assay agreed with those of conventional identification methods or with clinical data, showing that the test can be used for the direct and rapid detection and identification of mycobacteria in clinical samples.
Sensitivity of PCR Targeting the IS2404 Insertion Sequence of Mycobacterium ulcerans in an Assay Using Punch Biopsy Specimens for Diagnosis of Buruli Ulcer
Punch biopsy specimens from Mycobacterium ulcers disease lesions were used to compare the sensitivities and specificities of direct smear, culture, PCR, and histopathology in making a diagnosis of M. ulcerans disease in a field setting and the PCR was viable in a teaching hospital setting in Ghana.
Light emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis.
It is demonstrated that this form of illumination is suitable for the detection of auramine O stained Mycobacterium spp.