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Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface
TLDR
The real-time visualization of crystalline cellulose degradation by individual cellulase enzymes through use of an advanced version of high-speed atomic force microscopy is reported here.
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Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site
TLDR
The crystal structure of the M. albomyces laccase revealed elongated electron density between all three coppers in the trinuclear copper site, suggesting that an oxygen molecule binds with a novel geometry.
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Crystallographic evidence for substrate ring distortion and protein conformational changes during catalysis in cellobiohydrolase Ce16A from trichoderma reesei.
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High Speed Atomic Force Microscopy Visualizes Processive Movement of Trichoderma reesei Cellobiohydrolase I on Crystalline Cellulose*
TLDR
Real time observations of Trichoderma reesei (Tr) cellobiohydrolase I (Cel7A) molecules sliding on crystalline cellulose are presented with a high speed atomic force microscope and it is indicated that, besides the hydrolysis of glycosidic bonds, the loading of a cellulose chain into the active site tunnel is also essential for the enzyme movement.
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High level secretion of cellobiohydrolases by Saccharomyces cerevisiae
TLDR
Gene or protein specific features and compatibility with the host are important for efficient cellobiohydrolase secretion in yeast and production of both CBH1 and CBH2 could be improved to levels where the barrier to CBH sufficiency in the hydrolysis of cellulose was overcome.
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Lignocellulosic ethanol: from science to industry.
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Engineering the exo-loop of Trichoderma reesei cellobiohydrolase, Cel7A. A comparison with Phanerochaete chrysosporium Cel7D.
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Cello-oligosaccharide hydrolysis by cellobiohydrolase II from Trichoderma reesei. Association and rate constants derived from an analysis of progress curves.
The hydrolysis of soluble cello-oligosaccharides, with a degree of polymerisation of 4-6, catalysed by cellobiohydrolase II from Trichoderma reesei was studied using 1H-NMR spectroscopy and HPLC. The…
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Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose.
TLDR
This interaction was used to study the effect of different cellulose-binding domains (CBDs) on the enzymatic activity of C. thermocellum endoglucanase CelD, proving the reversible binding of the intact complexes despite the apparent binding irreversibility of some CBDs.
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Expression of Talaromyces emersonii cellobiohydrolase Cel7A in Saccharomyces cerevisiae and rational mutagenesis to improve its thermostability and activity.
TLDR
A successful expression of a single-module GH-7 family cellobiohydrolase Cel7A from a thermophilic fungus Talaromyces emersonii in Saccharomyces cerevisiae is reported, which shows, somewhat surprisingly, improved activity even at room temperature.
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