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Exploring polyethylenimine‐mediated DNA transfection and the proton sponge hypothesis
The relatively high transfection efficiency of polyethylenimine (PEI) vectors has been hypothesized to be due to their ability to avoid trafficking to degradative lysosomes. According to the proton…
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
- J. Agresti, E. Antipov, D. Weitz
- BiologyProceedings of the National Academy of Sciences
- 8 February 2010
This work presents a general ultrahigh-throughput screening platform using drop-based microfluidics that overcomes limitations and revolutionizes both the scale and speed of screening.
Designing surfaces that kill bacteria on contact
- J. Tiller, C. Liao, K. Lewis, A. Klibanov
- Materials Science, BiologyProceedings of the National Academy of Sciences…
- 15 May 2001
On surfaces modified with N-hexylated poly(4-vinylpyridine), the numbers of viable cells of another Gram-positive bacterium, Staphylococcus epidermidis, as well as of the Gram-negative bacteria Pseudomonas aeruginosa and Escherichia coli, dropped more than 100-fold compared with the original amino glass.
Improving enzymes by using them in organic solvents
- A. Klibanov
- Chemistry, MedicineNature
- 11 January 2001
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not only…
Peroxidase-Catalyzed Removal of Phenols from Coal-Conversion Waste Waters
A novel, enzymatic approach has been developed for the removal of phenols from coal-conversion aqueous effluents. Treatment with horseradish peroxidase and hydrogen peroxide precipitates 97 to 99…
Why are enzymes less active in organic solvents than in water?
- A. Klibanov
- BiologyTrends in biotechnology
- 1 March 1997
Enzymatic catalysis in nonaqueous solvents.
Visual Evidence of Acidic Environment Within Degrading Poly(lactic-co-glycolic acid) (PLGA) Microspheres
A pH gradient is shown, with the most acidic environment at the center of the spheres and higher pH near the edges, which is characteristic of diffusion-controlled release of the acidicdegradation products.
Stabilization of enzymes against thermal inactivation.
- A. Klibanov
- BiologyAdvances in applied microbiology
Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung.
- Mini Thomas, James J. Lu, Q. Ge, Chengcheng Zhang, Jianzhu Chen, A. Klibanov
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 19 April 2005
Systemic delivery in mice of the complexes of a short interfering RNA against a model gene, firefly luciferase, and PEI25 or PEI87 afforded a 77% and 93% suppression of the gene expression in the lungs, respectively, validation of the potential of such linear, fully deacylated PEIs in gene therapy for lung diseases.