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Exploring polyethylenimine‐mediated DNA transfection and the proton sponge hypothesis
The relatively high transfection efficiency of polyethylenimine (PEI) vectors has been hypothesized to be due to their ability to avoid trafficking to degradative lysosomes. According to the protonExpand
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Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
The explosive growth in our knowledge of genomes, proteomes, and metabolomes is driving ever-increasing fundamental understanding of the biochemistry of life, enabling qualitatively new studies ofExpand
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Designing surfaces that kill bacteria on contact
Poly(4-vinyl-N-alkylpyridinium bromide) was covalently attached to glass slides to create a surface that kills airborne bacteria on contact. The antibacterial properties were assessed by sprayingExpand
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Peroxidase-Catalyzed Removal of Phenols from Coal-Conversion Waste Waters
A novel, enzymatic approach has been developed for the removal of phenols from coal-conversion aqueous effluents. Treatment with horseradish peroxidase and hydrogen peroxide precipitates 97 to 99Expand
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Enzymatic catalysis in nonaqueous solvents.
Subtilisin and alpha-chymotrypsin vigorously act as catalysts in a variety of dry organic solvents. Enzymatic transesterifications in organic solvents follow Michaelis-Menten kinetics, and the valuesExpand
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Why are enzymes less active in organic solvents than in water?
  • A. Klibanov
  • Chemistry, Medicine
  • Trends in biotechnology
  • 1 March 1997
In order to exploit fully the biotechnological opportunities afforded by nonaqueous enzymology, the issue of often drastically diminished enzymatic activity in organic solvents compared with that inExpand
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Non-viral gene therapy: polycation-mediated DNA delivery
Gene therapy, i.e., the expression in cells of genetic material with therapeutic activity, holds great promise for the treatment of human diseases. A delivery vehicle (vector), of either viral orExpand
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Stabilization of enzymes against thermal inactivation.
  • A. Klibanov
  • Chemistry, Medicine
  • Advances in applied microbiology
  • 1983
Publisher Summary In order to be suitable for technological applications, catalysts should be stable under operational conditions for weeks or months. With continuous research it is found thatExpand
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The effect of water on enzyme action in organic media.
Three model, unrelated enzymes (yeast alcohol oxidase, mushroom polyphenol oxidase, and horse liver alcohol dehydrogenase) were found to be catalytically active in a variety of organic solvents. ForExpand
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