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Four mating‐type genes control sexual differentiation in the fission yeast.
The predicted product of the Pi gene contains a region of homology with the homeobox sequence, suggesting that this gene encodes a DNA binding protein that directly regulates the expression of other genes.
Cloning and characterization of four SIR genes of Saccharomyces cerevisiae.
Using the cloned SIR genes, it is shown that SIR3 at a high copy number is able to suppress mutations of SIR4 and RNA blot analysis suggests that this suppression is not due to transcriptional regulation of Sir3 by Sir4; nor does any S IR4 gene transcriptionally regulate another SIR gene.
Histone deacetylase homologs regulate epigenetic inheritance of transcriptional silencing and chromosome segregation in fission yeast.
The identification of an essential gene, clr6 (cryptic loci regulator), which encodes a putative histone deacetylase that when mutated affects epigenetically maintained repression at the mat2-mat3 region and at centromeres and reduces the fidelity of chromosome segregation is reported.
Three additional linkage groups that repress transcription and meiotic recombination in the mating-type region of Schizosaccharomyces pombe.
Evidence is presented that the transcription and recombination blocks of Schizosaccharomyces pombe require three newly defined trans-acting loci, clr2, clR3 and clr4, in addition to the previously identified clr1, rik1 and swi6 loci.
A recombinationally repressed region between mat2 and mat3 loci shares homology to centromeric repeats and regulates directionality of mating-type switching in fission yeast.
This work furthers the model that directionality is regulated by cell-type-specific organization of the heterochromatin-like structure in the mating-type region and provides evidence that the K-region contributes to silencing of the mat2-mat3 interval.
Statistical modeling and analysis of the LAGLIDADG family of site-specific endonucleases and identification of an intein that encodes a site-specific endonuclease of the HNH family.
Analysis of an HMM-generated multiple alignment of 130 LAGLIDADG family members and the three-dimensional structure of the I- Cre I endonuclease has enabled definition of the core elements of the repeated domain that is present in this family of proteins.
The chromo and SET domains of the Clr4 protein are essential for silencing in fission yeast
Results show that clr4 plays a critical role in silencing at mating-type loci and centromeres through the organization of repressive chromatin structure and demonstrate a new, activator function for Clr4p.