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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
TLDR
We revise and renew the “MISEV2014” guidelines for studies of extracellular vesicles based on evolution of the collective knowledge in the last four years. Expand
Rational siRNA design for RNA interference
Short-interfering RNAs suppress gene expression through a highly regulated enzyme-mediated process called RNA interference (RNAi). RNAi involves multiple RNA-protein interactions characterized byExpand
Functional siRNAs and miRNAs Exhibit Strand Bias
Abstract Both microRNAs (miRNA) and small interfering RNAs (siRNA) share a common set of cellular proteins (Dicer and the RNA-induced silencing complex [RISC]) to elicit RNA interference. In theExpand
Functional siRNAs and miRNAs Exhibit Strand Bias
Both microRNAs (miRNA) and small interfering RNAs (siRNA) share a common set of cellular proteins (Dicer and the RNA-induced silencing complex [RISC]) to elicit RNA interference. In the followingExpand
Analysis of Clathrin-mediated Endocytosis of Epidermal Growth Factor Receptor by RNA Interference*[boxs]
To identify proteins that participate in clathrin-mediated endocytosis of the epidermal growth factor receptor (EGFR), 13 endocytic proteins were depleted in HeLa cells using highly efficient smallExpand
Sequence elements outside the hammerhead ribozyme catalytic core enable intracellular activity
The hammerhead ribozyme (HHRz) is a small, naturally occurring ribozyme that site-specifically cleaves RNA and has long been considered a potentially useful tool for gene silencing. The minimalExpand
Identification of the gene for vitamin K epoxide reductase
Vitamin K epoxide reductase (VKOR) is the target of warfarin, the most widely prescribed anticoagulant for thromboembolic disorders. Although estimated to prevent twenty strokes per induced bleedingExpand
Position-specific chemical modification of siRNAs reduces "off-target" transcript silencing.
Transfected siRNAs regulate numerous transcripts sharing limited complementarity to the RNA duplex. This unintended ("off-target") silencing can hinder the use of RNAi to define gene function. HereExpand
Identification of genes that regulate epithelial cell migration using an siRNA screening approach
To provide a systematic analysis of genes that regulate epithelial cell migration, we performed a high throughput wound healing screen with MCF-10A breast epithelial cells, using siRNAs targetingExpand
3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets
Off-target gene silencing can present a notable challenge in the interpretation of data from large-scale RNA interference (RNAi) screens. We performed a detailed analysis of off-targeted genesExpand
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