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Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories
A number of PCR-based techniques can be used to detect polymorphisms in plants. For their wide-scale usage in germplasm characterisation and breeding it is important that these marker technologies… Expand
Microsatellite libraries enriched for several microsatellite sequences in plants.
Simple sequence repeats (SSRs), or microsatellites, have been isolated and characterized from numerous animal and plant species (6,7). These sequences have been shown to be highly polymorphic between… Expand
Molecular Techniques in the Assessment of Botanical Diversity
A variety of different molecular techniques can be used for the study of botanical diversity. Restriction fragment length polymorphism (RFLP), arbitrary primed DNA, amplified fragment length… Expand
Molecular tools for screening biodiversity: plants and animals
List of Contributors. Foreword M. Chase. Preface A. Karp, P.G. Isaac, D.S. Ingram. Introduction E. Magnien (EU DG XII). Part One: DNA Extraction. 1. DNA extraction: widely applicable methods. 2. DNA… Expand
A study of genetic diversity in Populus nigra subsp. betulifolia in the Upper Severn area of the UK using AFLP markers
A survey of amplified fragment length polymorphism (AFLP) was conducted to examine genetic diversity in 146 individuals of British, native black poplar (Populus nigra subsp. betulifolia) and three… Expand
A genetic linkage map of willow (Salix viminalis) based on AFLP and microsatellite markers
- S. Hanley, J. Barker, +5 authors A. Karp
- Biology, Medicine
- Theoretical and Applied Genetics
- 22 June 2002
Abstract.The genus Salix (willow) contains a number of species of great value as biomass crops. Efforts to breed varieties with improved biomass yields and resistances to pests and diseases are… Expand
An analysis of genetic diversity in coconut (Cocos nucifera) populations from across the geographic range using sequence-tagged microsatellites (SSRs) and AFLPs
Abstract Genetic diversity in 31 individuals from 14 coconut populations across the entire geographic range (2–3 individuals per population) was assessed using sequence-tagged microsatellites (or… Expand
Molecular technologies for biodiversity evaluation: Opportunities and challenges
- A. Karp, Keith J. Edwards, +9 authors Godfrey M. Hewitt
- Biology, Medicine
- Nature Biotechnology
- 1 July 1997
New technologies for detecting variation in DNA complement traditional methods in biodiversity.
Taxonomy, identification, genetic relationships and distribution of large Heracleum species in Europe.
Isolation and characterization of microsatellites in Echinochloa (L.) Beauv. spp.
Five primer pairs were developed that amplify microsatellite loci in three agronomically important Echinochloa (L.) Beauv. species: E. colona (L.) Link, E. crus-galli (L.) Beauv. and E. crus-pavonis… Expand