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High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells.
A scalable transfection procedure using polyethylenimine (PEI) is described for the human embryonic kidney 293 cell line grown in suspension. Green fluorescent protein (GFP) and human placentalExpand
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Growth, Nutrient Consumption, and End‐Product Accumulation in Sf‐9 and BTI‐EAA Insect Cell Cultures: Insights into Growth Limitation and Metabolism
Growth, nutrient consumption, and end‐product accumulation were quantitated in shake‐flask cultures of two insect cell lines, Sf‐9 and BTI‐EAA, in three different serum‐supplemented media. Per cellExpand
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Large-Scale transfection of mammalian cells for the fast production of recombinant protein
Recombinant proteins (r-proteins) are increasingly important in fundamental research and for clinical applications. As many of these r-proteins are of human or animal origin, cultivated mammalianExpand
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Transient gene expression in HEK293 cells: peptone addition posttransfection improves recombinant protein synthesis.
Gene expression by large-scale transfection of mammalian cells is becoming an established technology for the fast production of milligram and even gram amounts of recombinant proteins (r-proteins).Expand
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Production of lentiviral vectors by large‐scale transient transfection of suspension cultures and affinity chromatography purification
The use of lentiviral vectors as gene delivery vehicles has become increasingly popular in recent years. The growing interest in these vectors has created a strong demand for large volumes of vectorExpand
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Development of a scalable process for high‐yield lentiviral vector production by transient transfection of HEK293 suspension cultures
Lentiviral vectors (LV) offer several advantages over other gene delivery vectors. Their potential for the integration and long‐term expression of therapeutic genes renders them an interesting toolExpand
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Development and optimization of an adenovirus production process
Adenoviral vectors have a number of advantages such as their ability to infect post‐mitotic tissues. They are produced at high titers and are currently used in 28% of clinical protocols targetingExpand
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Insights into the Central Metabolism of Spodoptera frugiperda (Sf‐9) and Trichoplusia ni BTI‐Tn‐5B1‐4(Tn‐5) Insect Cells by Radiolabeling Studies
The insect cell baculovirus expression vector system (BEVS) is one of the most commonly used expression systems for recombinant protein production. This system is also widely used for the productionExpand
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A reporter gene assay for high-throughput screening of G-protein-coupled receptors stably or transiently expressed in HEK293 EBNA cells grown in suspension culture.
We describe in detail a robust, sensitive, and versatile functional assay for G-protein-coupled receptors (GPCRs) expressed in human embryonic kidney (HEK) 293-EBNA (Epstein-Barr virus nuclearExpand
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Production of adenovirus vector for gene therapy.
The field of gene therapy is rapidly expanding with a major focus on the treatment of cancer. Replication-defective adenoviruses are vectors of choice for delivering corrective genes into humanExpand
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