Structural basis for aminoglycoside inhibition of bacterial ribosome recycling
- M. A. Borovinskaya, R. Pai, J. Cate
- Biology, ChemistryNature Structural &Molecular Biology
- 29 July 2007
It is shown in X-ray crystal structures of the Escherichia coli 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal subunit, which is crucial for subunit association, to swing away from the subunit interface, providing a structural explanation for aminoglycoside inhibition of ribosomes recycling.
Translation initiation with 70S ribosomes: an alternative pathway for leaderless mRNAs.
In this study, it is demonstrated the proficiency of dedicated 70S ribosomes in in vitro translation of leaderless mRNAs, and it is shown that a natural leaderless mRNA can be translated with crosslinked 70S wild-type ribosome.
The role of ribosome recycling factor in dissociation of 70S ribosomes into subunits.
- G. Hirokawa, Romana M. Nijman, V. Raj, H. Kaji, K. Igarashi, A. Kaji
- Biology, Computer ScienceRNA: A publication of the RNA Society
- 1 August 2005
It is shown that 70S ribosomes, as well as the model post-termination complexes, are dissociated into stable subunits by cooperative action of three translation factors: ribosome recycling factor, elongation factor G (EF-G), and initiation factor 3 (IF3).
Crystal structure of Thermotoga maritima ribosome recycling factor: a tRNA mimic.
- M. Selmer, S. Al-Karadaghi, G. Hirokawa, A. Kaji, A. Liljas
- Biology, ChemistryScience
- 17 December 1999
The mimicry suggests that RRF interacts with the posttermination ribosomal complex in a similar manner to a tRNA, leading to disassembly of the complex.
Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: Functional implications
- R. Agrawal, Manjuli R. Sharma, J. Frank
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 3 June 2004
A three-dimensional cryo-electron microscopic map of a complex of the Escherichia coli 70S ribosome and RRF is obtained, finding that RRF interacts mainly with the segments of the large ribosomal subunit's (50S) rRNA helices that are involved in the formation of two central intersubunit bridges, B2a and B3.
Factor-dependent release of ribosomes from messenger RNA. Requirement for two heat-stable factors.
Role of elongation factor G and a protein factor on the release of ribosomes from messenger ribonucleic acid.
Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis
Ribosome recycling factor (RRF) catalyzes the fourth step of protein synthesis in vitro: disassembly of the post‐termination complex of ribosomes, mRNA and tRNA. We now report the first in vivo…
Ribosome recycling by ribosome recycling factor (RRF)--an important but overlooked step of protein biosynthesis.