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Structural basis for aminoglycoside inhibition of bacterial ribosome recycling
It is shown in X-ray crystal structures of the Escherichia coli 70S ribosome that RRF binding causes RNA helix H69 of the large ribosomal subunit, which is crucial for subunit association, to swing away from the subunit interface, providing a structural explanation for aminoglycoside inhibition of ribosomes recycling. Expand
Translation initiation with 70S ribosomes: an alternative pathway for leaderless mRNAs.
In this study, it is demonstrated the proficiency of dedicated 70S ribosomes in in vitro translation of leaderless mRNAs, and it is shown that a natural leaderless mRNA can be translated with crosslinked 70S wild-type ribosome. Expand
The role of ribosome recycling factor in dissociation of 70S ribosomes into subunits.
It is shown that 70S ribosomes, as well as the model post-termination complexes, are dissociated into stable subunits by cooperative action of three translation factors: ribosome recycling factor, elongation factor G (EF-G), and initiation factor 3 (IF3). Expand
Crystal structure of Thermotoga maritima ribosome recycling factor: a tRNA mimic.
The mimicry suggests that RRF interacts with the posttermination ribosomal complex in a similar manner to a tRNA, leading to disassembly of the complex. Expand
Role of elongation factor G and a protein factor on the release of ribosomes from messenger ribonucleic acid.
The hypothesis that elongation factor G and an additional factor play a role in the process of ribosome run-off from messenger RNA at the end of the cistron or abortively at the middle of thecistron is supported. Expand
Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis
Ribosome recycling factor (RRF) catalyzes the fourth step of protein synthesis in vitro: disassembly of the post‐termination complex of ribosomes, mRNA and tRNA. We now report the first in vivoExpand
Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: Functional implications
A three-dimensional cryo-electron microscopic map of a complex of the Escherichia coli 70S ribosome and RRF is obtained, finding that RRF interacts mainly with the segments of the large ribosomal subunit's (50S) rRNA helices that are involved in the formation of two central intersubunit bridges, B2a and B3. Expand
Factor-dependent release of ribosomes from messenger RNA. Requirement for two heat-stable factors.
It is postulated that the second factor, acting together with G factor, releases ribosomes from messenger RNA when the polypeptide chain is terminated naturally at the termination codons or abortively in the middle of cistron. Expand
Mitogen- and Stress-activated Protein Kinase 1 Mediates Activation of Akt by Ultraviolet B Irradiation*
Investigation of the mechanism by which UVB irradiation activates Akt in mouse epidermal JB6 cells suggested that UVB-induced Akt activation was mediated through Msk1, which is a downstream kinase of the Erk and p38 kinase signaling pathways. Expand