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Generation of human monoclonal antibodies against HIV-1 proteins; electrofusion and Epstein-Barr virus transformation for peripheral blood lymphocyte immortalization.
Electrofusion and EBV transformation were studied by immortalizing human PBLs from blood of HIV-1-positive volunteers. A panel of 33 cell lines producing human monoclonal antibodies (Hu-MAbs) againstExpand
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Protein Chromatography: Process Development and Scale-Up
Preface DOWNSTREAM PROCESSING OF BIOTECHNOLOGY PRODUCTS Introduction Bioproducts and their Contaminants Bioprocesses Role of Chromatography in Downstream Processing References INTRODUCTION TO PROTEINExpand
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A broadly neutralizing human monoclonal antibody against gp41 of human immunodeficiency virus type 1.
We have established a hybridoma clone, designated 2F5, secreting a neutralizing human monoclonal antibody (MAb) specific for gp41 of human immunodeficiency virus type 1 (HIV-1). The epitope of MAbExpand
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The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins.
A fusion tag, called FLAG and consisting of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. ItExpand
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Comparison of protein A affinity sorbents.
Protein A is a popular generic ligand for purification of monoclonal and recombinant antibodies. The performance of 15 commercially available protein A media was studied. Equilibrium and dynamicExpand
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Continuous downstream processing of biopharmaceuticals.
  • A. Jungbauer
  • Computer Science, Medicine
  • Trends in biotechnology
  • 1 August 2013
Continuous manufacturing has been applied in many different industries but has been pursued reluctantly in biotechnology where the batchwise process is still the standard. Expand
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Chromatographic media for bioseparation.
  • A. Jungbauer
  • Chemistry, Medicine
  • Journal of chromatography. A
  • 11 February 2005
Bioseparation processes are dominated by chromatographic steps. Even primary recovery is sometimes accomplished by chromatographic separation, using a fluidized bed instead of a fixed bed. In thisExpand
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Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies.
Monoliths are considered as the fourth-generation chromatography material. Their use for preparative separation of biomolecules has been evolved over the past decade. Monolithic columns up to 8L inExpand
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Current status of technical protein refolding.
The expression of heterologous proteins in microbial hosts frequently leads to the formation of insoluble aggregates. To fully exploit the production capacity of the cells, efficient strategies forExpand
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Npro fusion technology to produce proteins with authentic N termini in E. coli
We describe a prokaryotic expression system using the autoproteolytic function of Npro from classical swine fever virus. Proteins or peptides expressed as Npro fusions are deposited as inclusionExpand
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