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The release of genetically modified crops into the environment. Part II. Overview of ecological risk assessment.
TLDR
The increased knowledge base underpinning the development of GM crops will provide greater confidence in the assurances plant science can give on the risks of releasing such crops, and when assessing GM crops relative to existing cultivars.
pBINPLUS: An improved plant transformation vector based on pBIN19
TLDR
Improvements over pBIN19 include location of the selectable marker gene at the left T- DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.
Directed microspore-specific recombination of transgenic alleles to prevent pollen-mediated transmission of transgenes.
TLDR
The feasibility of engineering transgenic plants to produce pollen devoid of any transgene is shown, and this approach can prevent adventitious presence of transgenes in non-GM crops or related wild species by gene flow.
The MAR-Mediated Reduction in Position Effect Can Be Uncoupled from Copy Number-Dependent Expression in Transgenic Plants.
TLDR
DCaMV 35S-GUS gene expression in mature tobacco plants is regulated by some form of dosage compensation, and it is concluded that the selection bias for NPTII gene expression during transformation could not fully account for the lack of reduction in variation of NPT II gene expression.
The release of genetically modified crops into the environment. Part I. Overview of current status and regulations.
TLDR
The best and most appropriate baseline for comparison when performing risk assessment on GM crops is the impact of plants developed by traditional breeding, which is an integral and accepted part of agriculture.
Applications of biotechnology and genomics in potato improvement.
TLDR
The recent determination of the potato genome sequence has presented new opportunities for genomewide assays to provide tools for gene discovery and enabling the development of robustly unique marker haplotypes spanning QTL regions.
Identification of genes from the obligate intracellular plant pathogen, Plasmodiophora brassicae.
TLDR
A total of 76 new P. brassicae gene sequences were identified, the majority of which were extended to full length at the 5' end by the use of RACE amplification, and many of the unisequences were predicted to contain signal peptides for ER translocation.
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