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A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation
It is proposed that liquid-like compartments carry the trade-off between functionality and risk of aggregation and that aberrant phase transitions within liquid- like compartments lie at the heart of ALS and, presumably, other age-related diseases. Expand
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts
Functional nuclei and mitotic spindles are shown to assemble around DNA-coated beads incubated in Xenopus egg extracts, indicating that bipolarity is an intrinsic property of microtubules assembling around chromatin in a mitotic cytoplasm. Expand
Functional Analysis of Kinetochore Assembly in Caenorhabditis elegans
Different roles are suggested for the kinetochore and the chromosomal passengers in mitotic chromosome segregation and the recruitment of CeINCENP to chromosomes, which are independent of each other. Expand
Biomolecular condensates: organizers of cellular biochemistry
This work has shown that liquid–liquid phase separation driven by multivalent macromolecular interactions is an important organizing principle for biomolecular condensates and has proposed a physical framework for this organizing principle. Expand
Preparation of modified tubulins.
This chapter presents a collection of the various different ways by which tubulins are modified to generate probes for investigating microtubule (MT) dynamics in vitro and in vivo. Expand
Centriole assembly in Caenorhabditis elegans
Centrioles are necessary for flagella and cilia formation, cytokinesis, cell-cycle control and centrosome organization/spindle assembly. They duplicate once per cell cycle, but the mechanismsExpand
Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation
It is shown that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which is used to estimate their viscosity and surface tension, and reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell. Expand
A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances
This study provides a rich interaction dataset connecting thousands of proteins and introduces a framework for quantitative network analysis, revealing that the protein network is dominated by weak, substoichiometric interactions that play a pivotal role in defining network topology. Expand
Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans
This work used RNA-mediated interference to target 98% of all genes predicted in the C. elegans genome in combination with differential interference contrast time-lapse microscopy and developed a phenotypic profiling system, which shows high correlation with cellular processes and biochemical pathways, thus enabling to predict new functions for previously uncharacterized genes. Expand
Sororin Mediates Sister Chromatid Cohesion by Antagonizing Wapl
It is proposed that Sororin maintains cohesion by inhibiting Wapl's ability to dissociate cohesin from DNA, and that one of these, Dalmatian, is essential for cohesion in Drosophila. Expand