A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation
Biomolecular condensates: organizers of cellular biochemistry
- Salman F. Banani, Hyun O Lee, A. Hyman, M. Rosen
- BiologyNature reviews. Molecular cell biology
- 1 May 2017
This work has shown that liquid–liquid phase separation driven by multivalent macromolecular interactions is an important organizing principle for biomolecular condensates and has proposed a physical framework for this organizing principle.
Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation
It is shown that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which is used to estimate their viscosity and surface tension, and reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell.
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts
Functional nuclei and mitotic spindles are shown to assemble around DNA-coated beads incubated in Xenopus egg extracts, indicating that bipolarity is an intrinsic property of microtubules assembling around chromatin in a mitotic cytoplasm.
A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances
Functional Analysis of Kinetochore Assembly in Caenorhabditis elegans
Different roles are suggested for the kinetochore and the chromosomal passengers in mitotic chromosome segregation and the recruitment of CeINCENP to chromosomes, which are independent of each other.
Liquid-liquid phase separation in biology.
The basic physical concepts necessary to understand the consequences of liquid-like states for biological functions are discussed.
A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins
Preparation of modified tubulins.
Yeast kinesin-8 depolymerizes microtubules in a length-dependent manner
- Vladimír Varga, J. Helenius, Kozo Tanaka, A. Hyman, Tomoyuki U. Tanaka, J. Howard
- BiologyNature Cell Biology
- 13 August 2006
It is directly demonstrated that Kip3p destabilizes microtubules by depolymerizing them — accounting for the effects of kinesin-8 perturbations on microtubule and spindle length observed in fungi and metazoan cells.