Share This Author
Physiology and pathophysiology of sphingolipid metabolism and signaling.
SphK1 Regulates Proinflammatory Responses Associated with Endotoxin and Polymicrobial Sepsis
It is found that the enzyme sphingosine kinase 1 (SphK1) is up-regulated in stimulated human phagocytes and in peritoneal phagocyte of patients with severe sepsis and suggest that inhibition of SphK1 is a potential therapy for septic shock.
Inducible NO synthase: role in cellular signalling.
The discovery of endothelium-derived relaxing factor and its identification as nitric oxide (NO) was one of the most exciting discoveries of biomedical research in the 1980s. Besides its potent…
Posttranslational Modification of the AU-Rich Element Binding Protein HuR by Protein Kinase Cδ Elicits Angiotensin II-Induced Stabilization and Nuclear Export of Cyclooxygenase 2 mRNA
Using RNA interference technology and actinomycin D experiments, it is demonstrated that in human mesangial cells (hMC) the amplification of cytokine-induced COX-2 by angiotensin II (AngII) occurs via a HuR-mediated increase of mRNA stability.
Amplification of IL-1β-Induced Matrix Metalloproteinase-9 Expression by Superoxide in Rat Glomerular Mesangial Cells Is Mediated by Increased Activities of NF-κB and Activating Protein-1 and Involves…
- W. Eberhardt, A. Huwiler, K. Beck, S. Walpen, J. Pfeilschifter
- BiologyThe Journal of Immunology
- 15 November 2000
Exogenously administrated superoxide is identified as a costimulatory factor amplifying cytokine-induced MMP-9 expression by interfering with the signaling cascades leading to the activation of AP-1 and NF-κB.
Protein kinase C alpha-dependent phosphorylation of the mRNA-stabilizing factor HuR: implications for posttranscriptional regulation of cyclooxygenase-2.
- A. Doller, A. Huwiler, R. Müller, H. Radeke, J. Pfeilschifter, W. Eberhardt
- Biology, ChemistryMolecular biology of the cell
- 1 June 2007
RNA pull-down assay and RNA electrophoretic mobility shift assay demonstrated that the HuR shuttling by ATP is accompanied by an increased HuR binding to cyclooxygenase (COX)-2 mRNA.
Sphingosine 1-Phosphate Produced by Sphingosine Kinase 2 Intrinsically Controls Platelet Aggregation In Vitro and In Vivo.
It is demonstrated that Sphk2 is the major Sphk isoform responsible for the generation of S1P in platelets and plays a pivotal intrinsic role in the control of platelet activation.
Ceramide-binding and activation defines protein kinase c-Raf as a ceramide-activated protein kinase.
- A. Huwiler, J. Brunner, J. Pfeilschifter
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 9 July 1996
Protein kinase c-Raf is identified as a specific molecular target for interleukin 1 beta-stimulated ceramide formation and it is demonstrated that ceramide is a lipid cofactor participating in regulation of c- Raf activity.
The immunomodulatory sphingosine 1-phosphate analog FTY720 reduces lesion size and improves neurological outcome in a mouse model of cerebral ischemia.