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Comparison of fluorescent labels for oligosaccharides and introduction of a new postlabeling purification method.
2-aminopyridine was the label with the lowest retention on reversed-phase and graphitic carbon columns and appears to be most suitable for glycan fractionation by multidimensional HPLC, and most glycan derivatives performed better than native sugars in matrix-assisted laser desorption/ionization-mass spectrometry and electrospray ionization-MS. Expand
Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-glucose.
There is strong evidence that the elucidated biosynthesis pathway of dTDP-alpha-D-Quip3NAc may be valid for LPS (lipopolysaccharide) O-antigen structures and antibiotic precursors. Expand
Convergence rate for the Bayesian approach to linear inverse problems
Recently, the metrics of Ky Fan and Prokhorov were introduced as a tool for studying convergence of regularization methods for stochastic ill-posed problems. In this work, we show that the BayesianExpand
Cellulose solutions in N-methylmorpholine-N-oxide (NMMO) – degradation processes and stabilizers
Efficient stabilization of cellulose solutions in NMMO(1) against side reactions and their harmful effects meansprevention of both homolytic and heterolytic side reactions, which isExpand
On the conformation of the cellulose solvent N-methylmorpholine-N-oxide (NMMO) in solution
Abstract The N-oxide group of the cellulose solvent N-methylmorpholine-N-oxide showed a strong preference for the axial position compared with N-methyl as determined by NMR experiments andExpand
Biosynthesis of dTDP-3-acetamido-3,6-dideoxy-α-D-galactose in Aneurinibacillus thermoaerophilus L420-91T*
The key enzyme in the biosynthesis of dTDP-d-Fucp3NAc has been identified as an isomerase, which converts the 4-keto educt into the 3-ketO product, with concomitant epimerization at C-4 to produce a 6-deoxy-d -xylo configuration. Expand
Genome Analysis and Characterisation of the Exopolysaccharide Produced by Bifidobacterium longum subsp. longum 35624™
Data demonstrate that the B. longum 35624 strain has specific genetic features, one of which leads to the generation of a characteristic exopolysaccharide, which is a well described probiotic with clinical efficacy in Irritable Bowel Syndrome clinical trials and induces immunoregulatory effects in mice and in humans. Expand
Molecular Basis of S-layer Glycoprotein Glycan Biosynthesis in Geobacillus stearothermophilus*
The Gram-positive bacterium Geobacillus stearothermophilus NRS 2004/3a possesses a cell wall containing an oblique surface layer (S-layer) composed of glycoprotein subunits. O-Glycans with theExpand
UDP-xylose and UDP-galactose synthesis in Trichomonas vaginalis
The genes encoding the enzymes required for UDP-xylose and UDP-galactose synthesis in Trichomonas vaginalis have been identified and the products of the recombinant enzymes analysed. Expand
Convergence Rates for Linear Inverse Problems in the Presence of an Additive Normal Noise
Abstract In this work, we examine a finite-dimensional linear inverse problem where the measurements are disturbed by an additive normal noise. The problem is solved both in the frequentist and inExpand