Effects of Refractive Index and Viscosity on Fluorescence and Anisotropy Decays of Enhanced Cyan and Yellow Fluorescent Proteins
Time-resolved fluorescence measurements of the enhanced forms of ECFP and EYFP in water–glycerol mixtures were performed to quantify the effects of the refractive index and viscosity on the fluorescence lifetimes of these proteins.
Flexibility of Enzymes Suspended in Organic Solvents Probed by Time-Resolved Fluorescence Anisotropy. Evidence That Enzyme Activity and Enantioselectivity Are Directly Related to Enzyme Flexibility
- J. Broos, A. Visser, J. Engbersen, W. Verboom, A. Hoek, D. Reinhoudt
- Chemistry, Biology
- 1 December 1995
The relationship between the intemal rotational mobility of the fluorophore and the enantioselectivity provides the first experimental evidence that enzyme flexibility and enzyme enantiOSElectivity are correlated.
Intramolecular interactions in the ground and excited state of tetrakis(N-methylpyridyl)porphyrins.
The fluorescent properties of the cationic free base tetrakis(4-N-methylpyridy1)porphyrin (H2TMPyP(4)) in aqueous solution have been the subject of considerable discussion. Conclusions by various…
Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
For analysis of the time-resolved fluorescence experiments of VFPs, the maximum entropy method procedure is utilised to obtain a distribution of fluorescence lifetimes and distributed lifetime patterns turn out to have diagnostic value, for instance, in observing populations of V FP pairs that are FRET-inactive.
Visualization of BRI 1 and BAK 1 ( SERK 3 ) membrane receptor hetero-oligomers during brassinosteroid signaling
It is proposed that only small populations of PMlocated BRI 1 and BAK1(SERK3) receptors participate in active BR signaling and that the initiation of downstream signal transduction involves preassembled BRI1-BAK1(serk3) heterooligomers.
Time‐Resolved Fluorescence Study of the Dissociation of FMN from the Yellow Fluorescence Protein from Vibrio fischeri
Time-resolved fluorescence spectroscopy of the flavin mononucleotide (FMN) prosthetic group of the yellow fluorescence protein (YFP) from Vibrio @hen has provided quantitative, thermodynamic…
THE FLUORESCENCE DECAY OF REDUCED NICOTINAMIDES IN AQUEOUS SOLUTION AFTER EXCITATION WITH A UV‐MODE LOCKED Ar ION LASER
Abstract— The fluorescence decay kinetics of the reduced nicotinamides NMNH, NADH and NADPH in aqueous solution were investigated using an Ar ion laser, mode locked in the UV, as source of excitation…
Structure and dynamics of egg white ovalbumin adsorbed at the air/water interface
- E. Kudryashova, M. Meinders, A. Visser, A. Hoek, H. Jongh
- ChemistryEuropean Biophysics Journal
- 23 April 2003
The molecular properties of egg white ovalbumin adsorbed at the air/water interface were studied using infrared reflection absorption spectroscopy and time-resolved fluorescence anisotropy techniques and indicate that the end-over-end mobility of the ellipsoidal protein becomes substantially restricted.
Pulse selection system with electro‐optic modulators applied to mode‐locked cw lasers and time‐resolved single photon counting
An electro‐optical pulse selection system for mode‐locked cw lasers is described. The repetition rate of the exciting pulses can be chosen by electronically dividing the mode locker drive frequency.…
Dynamics of phospholipid molecules in micelles : Characterization with fluorescence corellation spectroscopy and time-resolved fluorescence anisotropy
The dynamic properties of two BODIPY labeled phospholipid molecules in micellar systems have been studied with two different spectroscopic techniques, which provide complementary information…