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Effects of Refractive Index and Viscosity on Fluorescence and Anisotropy Decays of Enhanced Cyan and Yellow Fluorescent Proteins
Time-resolved fluorescence measurements of the enhanced forms of ECFP and EYFP in water–glycerol mixtures were performed to quantify the effects of the refractive index and viscosity on the fluorescence lifetimes of these proteins.
Flexibility of Enzymes Suspended in Organic Solvents Probed by Time-Resolved Fluorescence Anisotropy. Evidence That Enzyme Activity and Enantioselectivity Are Directly Related to Enzyme Flexibility
A time-resolved fluorescence anisotropy study on the molecular flexibility of active-site labeled anthraniloyl-α-chymotrypsin, dansylsubtilisin Carlsberg, and native subtilisin Carlsberg, suspended
High-affinity binding of very-long-chain fatty acyl-CoA esters to the peroxisomal non-specific lipid-transfer protein (sterol carrier protein-2).
Binding of fluorescent fatty acids to bovine liver non-specific lipid-transfer protein (nsL-TP) was assessed by measuring fluorescence resonance energy transfer (FRET) between the single tryptophan
Time‐Resolved Fluorescence Study of the Dissociation of FMN from the Yellow Fluorescence Protein from Vibrio fischeri
Time-resolved fluorescence spectroscopy of the flavin mononucleotide (FMN) prosthetic group of the yellow fluorescence protein (YFP) from Vibrio @hen has provided quantitative, thermodynamic
Intramolecular interactions in the ground and excited state of tetrakis(N-methylpyridyl)porphyrins.
The fluorescent properties of the cationic free base tetrakis(4-N-methylpyridy1)porphyrin (H2TMPyP(4)) in aqueous solution have been the subject of considerable discussion. Conclusions by various
Pulse selection system with electro‐optic modulators applied to mode‐locked cw lasers and time‐resolved single photon counting
An electro‐optical pulse selection system for mode‐locked cw lasers is described. The repetition rate of the exciting pulses can be chosen by electronically dividing the mode locker drive frequency.
Structure and dynamics of egg white ovalbumin adsorbed at the air/water interface
The molecular properties of egg white ovalbumin adsorbed at the air/water interface were studied using infrared reflection absorption spectroscopy and time-resolved fluorescence anisotropy techniques and indicate that the end-over-end mobility of the ellipsoidal protein becomes substantially restricted.
Ultrafast resonance energy transfer from a site-specifically attached fluorescent chromophore reveals the folding of the N-terminal domain of CP29
The photosynthetic minor antenna complex CP29 of higher plants was singly mutated, overexpressed in Escherichia coli, selectively labeled with the fluorescent dye TAMRA at three positions in the
Time-resolved FRET fluorescence spectroscopy of visible fluorescent protein pairs
For analysis of the time-resolved fluorescence experiments of VFPs, the maximum entropy method procedure is utilised to obtain a distribution of fluorescence lifetimes and distributed lifetime patterns turn out to have diagnostic value, for instance, in observing populations of V FP pairs that are FRET-inactive.