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Microprocessor, Setx, Xrn2, and Rrp6 Co-operate to Induce Premature Termination of Transcription by RNAPII
Transcription elongation is increasingly recognized as an important mechanism of gene regulation. Here, we show that microprocessor controls gene expression in an RNAi-independent manner.Expand
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The HIV-1 Tat Protein Has a Versatile Role in Activating Viral Transcription
ABSTRACT It is generally acknowledged that the Tat protein has a pivotal role in HIV-1 replication because it stimulates transcription from the viral long terminal repeat (LTR) promoter by binding toExpand
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Construction of a Doxycycline-Dependent Simian Immunodeficiency Virus Reveals a Nontranscriptional Function of Tat in Viral Replication
ABSTRACT In the quest for an effective vaccine against human immunodeficiency virus (HIV), live attenuated virus vaccines have proven to be very effective in the experimental model system of simianExpand
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Tat-dependent production of an HIV-1 TAR-encoded miRNA-like small RNA
Evidence is accumulating that retroviruses can produce microRNAs (miRNAs). To prevent cleavage of their RNA genome, retroviruses have to use an alternative RNA source as miRNA precursor. TheExpand
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Mechanistic insights on the Dicer-independent AGO2-mediated processing of AgoshRNAs
Short hairpin RNAs (shRNAs) are widely used for gene knockdown by inducing the RNA interference (RNAi) mechanism, both for research and therapeutic purposes. The shRNA precursor is processed by theExpand
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Silencing of HIV-1 by AgoshRNA molecules
RNA interference (RNAi) is a sequence-specific gene silencing mechanism that is triggered by the expression of a short hairpin RNA (shRNA). shRNA molecules enter the RNAi pathway at the DicerExpand
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Deep sequencing of small TAR-derived RNAs in HIV-1 producing cells
were derived from the 3’side of the TAR stem. This set of TAR-derived small RNAs overlapped considerably, but varied in size (14-21 nt). In the HIV-rtTA samples, a significantly larger fractionExpand
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HIV-1 transcriptional silencing caused by TRIM22 inhibition of Sp1 binding to the viral promoter
BackgroundIntracellular defense proteins, also referred to as restriction factors, are capable of interfering with different steps of the viral life cycle. Among these, we have shown that TripartiteExpand
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Probing the shRNA characteristics that hinder Dicer recognition and consequently allow Ago-mediated processing and AgoshRNA activity.
Recent evidence indicates the presence of alternative pathways for microRNA (miRNA) and short hairpin (shRNA) processing. Specifically, some of these molecules are refractory to Dicer-mediatedExpand
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Retroviral microRNAs.
Eukaryotic cells and several DNA viruses encode miRNAs to regulate the expression of specific target genes. It has been controversial whether RNA viruses can encode such miRNAs as miRNA excision mayExpand
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