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Encapsidated Conformation of Bacteriophage T7 DNA
The structural organization of encapsidated T7 DNA was investigated by cryo-electron microscopy and image processing. A tail-deletion mutant was found to present two preferred views of phage heads:Expand
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Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
Plasmid vectors are described that allow cloning of target DNAs at sites where they will be minimally transcribed by Escherichia coli RNA polymerase but selectively and actively transcribed by T7 RNAExpand
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Cloning and expression of the gene for bacteriophage T7 RNA polymerase.
The complete coding sequence of the gene for bacteriophage T7 RNA polymerase (T7 gene 1) has been cloned in the plasmid pBR322. Large amounts of active enzyme can be accumulated in Escherichia coliExpand
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Biochemical Analysis of Mutant T7 Primase/Helicase Proteins Defective in DNA Binding, Nucleotide Hydrolysis, and the Coupling of Hydrolysis with DNA Unwinding*
We characterized nine helicase-deficient mutants of bacteriophage T7 helicase-primase protein (4A′) prepared by random mutagenesis as reported in the accompanying paper (Rosenberg, A. H., Griffin,Expand
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Cloning and expression of the gene for bacteriophage T 7 RNA polymerase
The complete coding sequence of the gene for bacteriophage T7 RNA polymerase (T7 gene 1) has been cloned in the plasmid pBR322. Large amounts of active enzyme can be accumulated in Escherichia coliExpand
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Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli.
Insertion of nine consecutive low-usage CUA leucine codons after codon 13 of a 313-codon test mRNA strongly inhibited its translation without apparent effect on translation of other mRNAs containingExpand
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Genetic and physical mapping in the early region of bacteriophage T7 DNA.
Abstract A detailed physical map of the early region of bacteriophage T7 DNA has been constructed. This map contains: locations for all the cuts made by the restriction endonucleases Hin dII, Hpa II,Expand
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Utilization of bacteriophage T7 late promoters in recombinant plasmids during infection.
Abstract When bacteriophage T7 infects a cell that carries a recombinant plasmid having a promoter for T7 RNA polymerase, the cloned promoter is utilized in the plasmid. A simple hybridization testExpand
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Genetic and physical mapping of the late region of bacteriophage T7 DNA by use of cloned fragments of T7 DNA.
Abstract Specific fragments of bacteriophage T7 DNA that account for about 99% of the total molecule have been cloned in the plasmid pBR322. This set of plasmids was used to map individual pointExpand
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