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Liver tissue engineering: a role for co-culture systems in modifying hepatocyte function and viability.
The potential of 3T3 fibroblast cells in a co-culture system to modulate function and viability of primary isolated rat hepatocytes and the hepatocytes within the co-cultures maintained viability, possessed well-formed canalicular systems, and displayed both functional markers. Expand
Effect of serum-free medium on cytochrome P450-dependent metabolism and toxicity in rat cultured hepatocytes.
The observed increase in toxicity is consistent with the improved maintenance of P450 in hepatocytes cultured in serum-free medium, although there is still a selective decline in P450 activities and toxicity with increased time in culture. Expand
Effects of culture duration, cytochrome P-450 inhibition and glutathione depletion on toxicity of diverse xenobiotics.
  • A. H. Hammond, J. Fry
  • Chemistry, Medicine
  • Toxicology in vitro : an international journal…
  • 1 June 1996
For the compounds tested, culture age seems less important in determining toxicity than choice of glutathione-depleting agent. Expand
Report of the International Workshop on In Vitro Methods for Assessing Acute Systemic Toxicity
Toxicity testing is conducted to determine the potential human health hazards of chemicals and products. Acute systemic toxicity testing is used to properly classify and appropriately label materialsExpand
Toxicity of dichloropropanols in rat hepatocyte cultues.
It is concluded that the toxicity of 1,3-dichloropropanol is mediated by cytochrome P450 and involves depletion of glutathione and loss of mitochondrial function. Expand
Comparison of in vivo and in vitro rat hepatic toxicity of coumarin and methyl analogues, and application of quantitative morphometry to toxicity in vivo.
It was determined that there was a lobar variation in the extent of hepatic damage but that this exhibited random inter-animal variation, and the order of cytotoxicity in vitro was identical to that observed in vivo. Expand
A Preliminary Comparison of LiverBeads™ with a Conventional Rat Hepatocyte Culture Preparation: Some Aspects of Xenobiotic Metabolism and Related Toxicity
Overall, in terms of the aspects of drug metabolism measured, the cultures from LiverBeads appeared to be equivalent to conventional cultures, and superior to cultures from cryopreserved cells. Expand
Competing pathways in metabolism-mediated cytotoxicity in vitro.
Results of recent studies on the cytotoxicity of cyclophosphamide, bromobenzene and paracetamol measured in a liver homogenate fraction-cultured cell co-incubation have confirmed the importance of activation/inactivation balance and stability of the reactive metabolite as determinants of metabolism-mediated cytot toxicity in vitro. Expand
Toxicity of coumarin and various methyl derivatives in cultures of rat hepatocytes and V79 cells.
The data obtained are consistent with hepatocyte toxicity being due to the cytochrome P-450-dependent formation of one or more toxic metabolites that may be detoxified by reacting with GSH, although depletion of GSH levels significantly increased the hepatocytes toxicity of both compounds. Expand
The in vivo induction of rat hepatic cytochrome P450-dependent enzyme activities and their maintenance in culture.
The feasibility of an in vivo induction-hepatocyte culture system for the study of metabolism-mediated toxicity is indicated. Expand