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A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
TLDR
This work presents a therapy-grade, inexpensive and simple protocol to derive MSCs from pluripotent stem cells (PSCs) based on the use of platelet lysate (PL) as medium supplement and shows that it can be used to generate a large amount of PD-MSCs with low cost and is compatible with clinical therapies. Expand
Hepatic Nuclear Factor 3 and Nuclear Factor 1 Regulate 5-Aminolevulinate Synthase Gene Expression and Are Involved in Insulin Repression*
TLDR
A model in which insulin exerts its negative effect through the disturbance of HNF3β binding or transactivation potential, probably due to specific phosphorylation of this transcription factor by Akt is proposed. Expand
Phosphatidylinositol 3-kinase and Ras/mitogen-activated protein kinase signaling pathways are required for the regulation of 5-aminolevulinate synthase gene expression by insulin.
TLDR
Factors involved in two signaling pathways that are often considered to be functionally separate during insulin action, the Ras/ERK/p90(RSK) pathway and the PI3K/PKB pathway, are jointly required for insulin-mediated inhibition of ALAS gene expression in rat hepatocytes and human hepatoma cells. Expand
Inhibitory Effect of AP-1 Complex on 5-Aminolevulinate Synthase Gene Expression through Sequestration of cAMP-response Element Protein (CRE)-binding Protein (CBP) Coactivator*
TLDR
It is demonstrated that c-Fos, c-Jun, and JunD are involved in TPA inhibitory effect due to their ability to bind TRE-ALAS, evidenced by supershift analysis and their capacity to repress promoter activity in transfection assays. Expand
AKT/GSK3β signaling pathway is critically involved in human pluripotent stem cell survival
TLDR
It is demonstrated by pharmacological inhibition and siRNA knockdown that GSK3β signaling is responsible, at least in part, of the apoptosis triggered by AKT inhibition, which decreases basal apoptosis rate and promotes PSC proliferation. Expand
EDA-Containing Fibronectin Increases Proliferation of Embryonic Stem Cells
TLDR
It is shown for the first time that this FN isoform enhances ESC’s proliferation, suggesting a possible conserved behavior for regulation of ES cells proliferation by this FNisoform and could contribute to improve their culturing conditions both for research and cell therapy. Expand
Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells.
TLDR
A deeper knowledge of the epigenetic regulation of ESC will allow improving somatic cell reprogramming for iPSC obtention and differentiation protocols optimization, and could contribute to the comprehension of molecular mechanisms involved in pluripotency, early differentiation and reprograming. Expand
Deep Learning Neural Networks Highly Predict Very Early Onset of Pluripotent Stem Cell Differentiation
TLDR
It is found that the networks can be trained to recognize undifferentiated cells from differentiating cells with an accuracy higher than 99% and displayed great performance in several similar pluripotent stem cell (PSC) settings, including mesoderm differentiation in human induced PSCs. Expand
Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2.
TLDR
Findings suggest that besides its known function in nervous system, Prmt8 could play a role in pluripotent stem cells, and its transcription is modulated by pluripotency transcription factors. Expand
Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
TLDR
Findings suggest that during differentiation, compared with their parental iPSC EVs, PD-MSC EVs acquire a more specific set of proteins; arguably, this difference might confer their therapeutic properties. Expand
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