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Branching Microtubule Nucleation in Xenopus Egg Extracts Mediated by Augmin and TPX2
It is demonstrated that microtubule nucleation from the sides of existing microtubules in meiotic Xenopus egg extracts is well suited for spindle assembly and maintenance. Expand
Evidence for an Upper Limit to Mitotic Spindle Length
It is concluded that early mitotic spindle length in Xenopus laevis is uncoupled from cell length, reaching an upper bound determined by mechanisms that are intrinsic to the spindle. Expand
Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles.
The response to microtubule destabilization suggests that an unidentified tensile element acts in parallel with these conventional factors, generating spindle shortening force. Expand
The kinesin Eg5 drives poleward microtubule flux in Xenopus laevis egg extract spindles
The results suggest that ensembles of nonprocessive Eg5 motors drive flux in metaphase Xenopus extract spindles, and pharmacological inhibition of Eg5 results in a dose–responsive slowing of flux, and biochemical depletion of Eg 5 significantly decreases the flux rate. Expand
The BRCA1/BARD1 Heterodimer Modulates Ran-Dependent Mitotic Spindle Assembly
It is shown that, in both mammalian cells and Xenopus egg extracts, BRCA1/BARD1 is required for mitotic spindle-pole assembly and for accumulation of TPX2, a major spindle organizer and Ran target, on spindle poles. Expand
XRHAMM Functions in Ran-Dependent Microtubule Nucleation and Pole Formation during Anastral Spindle Assembly
The Xenopus ortholog of human RHAMM is identified as a microtubule-associated protein that plays a role in focusing spindle poles and is essential for efficient micro Tubule nucleation during spindle assembly without centrosomes. Expand
The NIMA-family kinase Nek6 phosphorylates the kinesin Eg5 at a novel site necessary for mitotic spindle formation
It is shown that Nek6 is constitutively associated with Eg5, a kinesin that is necessary for spindle bipolarity, and during mitosis Nek6 phosphorylates a subset of Eg5 polypeptides at a conserved site, the phosphorylation of which is crucial for the mitotic function of Eg 5. Expand
Active Nercc1 protein kinase concentrates at centrosomes early in mitosis and is necessary for proper spindle assembly.
XNercc contributes to both the centrosomal and the chromatin/Ran pathways that collaborate in the organization of a bipolar spindle, suggesting that active Nercc has important functions at the microtubular organizing center during cell division. Expand
In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation.
The yeast actin-regulating kinases Ark1p and Prk1p are signaling proteins localized to cortical actin patches, which may be sites of endocytosis. Interactions between the endocytic proteins Pan1p andExpand
How does a millimeter-sized cell find its center?
Current understanding of microtubules organization during the first cell cycle in a fertilized Xenopus egg is summarized, how microtubule asters move towards the center of this very large cell is discussed, and how multiple asters shape and position themselves relative to each other are discussed. Expand