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By-passing immunization. Human antibodies from V-gene libraries displayed on phage.
TLDR
The results suggest that a single large phage display library can be used to isolate human antibodies against any antigen, by-passing both hybridoma technology and immunization. Expand
Phage antibodies: filamentous phage displaying antibody variable domains
TLDR
It is shown that complete antibody V domains can be displayed on the surface of fd bacteriophage, that the phage bind specifically to antigen and that rare phage can be isolated after affinity chromatography. Expand
Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.
TLDR
Heterodimeric Fab fragments can be assembled on the surface of the phages by linking one chain to the phage coat protein, and secreting the other into the bacterial periplasm by introducing an amber mutation. Expand
Making antibody fragments using phage display libraries
TLDR
Using a random combinatorial library of the rearranged heavy and kappa light chains from mice immune to the hapten 2-phenyloxazol-5-one (phOx), diverse libraries of antibody fragments are displayed on the surface of fd phage and elicited many more pairings with strong binding activities. Expand
Man-made cell-like compartments for molecular evolution
TLDR
It is demonstrated the linkage of genotype to phenotype in man-made compartments using a model system and a selection for target-specific DNA methylation was based on the resistance of the product (methylated DNA) to restriction digestion. Expand
Making antibodies by phage display technology.
TLDR
Human antibody fragments with many different binding specificities have been isolated from the same phage repertoire, including haptens, carbohydrates, secreted and cell surface proteins, viral coat proteins, and intracellular antigens from the lumen of the endoplasmic reticulum and the nucleus. Expand
Multiplex picodroplet digital PCR to detect KRAS mutations in circulating DNA from the plasma of colorectal cancer patients.
TLDR
Clinical utility of multiplex dPCR to screen for multiple mutations simultaneously with a sensitivity sufficient to detect mutations in circulating DNA obtained by noninvasive blood collection is demonstrated. Expand
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
TLDR
This work presents a general ultrahigh-throughput screening platform using drop-based microfluidics that overcomes limitations and revolutionizes both the scale and speed of screening. Expand
Quantitative and sensitive detection of rare mutations using droplet-based microfluidics.
TLDR
The technique enabled the determination of mutant allelic specific imbalance (MASI) in several cancer cell-lines and the precise quantification of a mutated KRAS gene in the presence of a 200,000-fold excess of unmutated KRAS genes. Expand
Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli
TLDR
Isolated variable domains may offer an alternative to monoclonal antibodies and serve as the key to building high-affinity human antibodies and the name 'single domain antibodies (dAbs)' is suggested for these antigen binding demands. Expand
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