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The Effect of organic nitrogen sources on recombinant glucoamylase production by Aspergillus niger in chemostat culture.
In cultures supplemented with l-alanine, l-methionine, casamino acids, or peptone, specific glucoamylase (GAM) production rapidly decreased to less than 20% of the initial level, although few had lost copies of the glaA gene.
Restriction fragment length polymorphisms in mitochondrial DNA and ribosomal RNA gene complexes as an aid to the characterization of species and sub-species populations in the genus Verticillium
A combination of mitochondrial and ribosomal RNA gene complex RFLPs has potential as an aid for the characterization of species and sub-species populations in the genes Verticillium.
Ribosomal, mitochondrial and amplified DNA polymorphisms in Verticillium albo-atrum pathogenic to hops, lucerne and other plants
Polymorphisms in mitochondrial DNA were detected in ethidium bromide stained gels after digestion of total genomic DNA with restriction enzymes which recognize four bases containing only G and C, indicating the genetic differentiation between hop and lucerne isolates.
Cell banking for pharmaceutical research.
Extracellular proteases produced by the Quorn® myco-protein fungus Fusarium graminearum in batch and chemostat culture.
For all concentrations of casein examined, protease activity was greater in chemostat culture than in batch culture; however, as the cultures passed from late exponential into stationary phase, the pH profile of the prote enzyme activity broadened until most of it was in the alkaline pH region.
Protease‐deficient mutants of the Quorn® mycoprotein fungus, Fusarium graminearum A3/5
Protease-deficient mutants of two highly branched (colonial) mutants of the Quorn® mycoprotein fungus, Fusarium graminearum A3/5, were generated by UV mutagenesis and were found to produce no or very little aspartic protease, which is produced by the parental strains under the same conditions.
Cloning and characterization of the ribosomal RNA gene complex from the plant pathogen Verticillium albo-atrum
The DNA coding for the ribosomal RNA gene complex (rDNA) has been cloned from isolate 621P(PV1) of Verticillium albo-atrum which is pathogenic for hops (Humulus lupulus). The rDNA was mapped using a