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NADP-Glutamate Dehydrogenase Isoenzymes of Saccharomyces cerevisiae
- A. DeLuna, A. Avendaño, L. Riego, A. González
- Biology, EngineeringThe Journal of Biological Chemistry
- 23 November 2001
The results indicate that the coordinated regulation of GDH1, GDH3, andGDH2-encoded enzymes results in glutamate biosynthesis and balanced utilization of α-ketoglutarate under fermentative and respiratory conditions.
GDH3 encodes a glutamate dehydrogenase isozyme, a previously unrecognized route for glutamate biosynthesis in Saccharomyces cerevisiae
- A. Avendaño, A. DeLuna, H. Olivera, L. Valenzuela, A. González
- Biology, EngineeringJournal of bacteriology
- 1 September 1997
The results indicate that GDH3 plays a significant physiological role, providing glutamate when GDH1 and GLT1 are impaired, the first example of a microorganism possessing three pathways for glutamate biosynthesis.
Lactobacillus plantarum phytase activity is due to non‐specific acid phosphatase
Microbial phytases suitable for food fermentations could be obtained from lactic acid bacteria isolated from natural vegetable fermentations thanks to a non‐specific acid phosphatase which had a higher hydrolysis rate with monophosphorylated compounds such as acetyl phosphate that could explain the low phytase activity.
Specialization of the paralogue LYS21 determines lysine biosynthesis under respiratory metabolism in Saccharomyces cerevisiae.
It can be concluded that retention and further biochemical specialization of the LYS20- and LYS21-encoded enzymes with partially overlapping roles contributed to the acquisition of facultative metabolism.
Saccharomyces cerevisiae has a single glutamate synthase gene coding for a plant-like high-molecular-weight polypeptide
The results show that S. cerevisiae has a single NADH-GOGAT enzyme, consisting of three 199-kDa monomers, that differs from the one found in prokaryotic microorganisms but is similar to those found in other eukaryotic organisms such as alfalfa.
The mtmVUC genes of the mithramycin gene cluster in Streptomycesargillaceus are involved in the biosynthesis of the sugar moieties
A pathway is proposed for the biosynthesis of the three sugar moieties in mithramycin, and it is suggested that the glycosyltransferase responsible for the incorporation of D-olivose shows some degree of flexibility with respect to its sugar co-substrate, since the 4-keto-analog is also transferred.
Sequence of the GLT1 gene from Saccharomyces cerevisiae reveals the domain structure of yeast glutamate synthase
- P. Filetici, M. P. Martegani, L. Valenzuela, A. González, P. Ballario
- Biology, ChemistryYeast
- 1 October 1996
The GOGat enzyme has been purified and the GOGAT structural gene (GLT1) has been cloned, showing that this enzyme is a homotrimeric protein with a monomeric size of 199kDa.
Neurospora crassa mutant impaired in glutamine regulation
It is proposed that the lower repression of glutamine synthetase by glutamine in this mutant could be a necessary condition for sustaining the higher flow of nitrogen for the accumulation of amino acids observed in ammonium excess and that, if glutamine is the nitrogen signal that regulates the arginine accumulation of the vesicle, the glnr mutant has also escaped this control.
The bromodomain of Gcn5p interacts in vitro with specific residues in the N terminus of histone H4.
- P. Ornaghi, P. Ballario, A. Lena, A. González, P. Filetici
- Biology, ChemistryJournal of molecular biology
- 19 March 1999
A glutathione S-transferase pull down assay is used to show that Gcn5p binds the amino-terminal tails of histones H3 and H4, but not H2A and H2B, and suggests a new structural role for the highly evolutionary conserved bromodomain.
Cysteine-scanning mutagenesis of helix IV and the adjoining loops in the lactose permease of Escherichia coli: Glu126 and Arg144 are essential. off.
The results indicate that the cytoplasmic loop between helices IV and V, where insertional mutagenesis has little effect on activity, contains residues that play an important role in permease activity and that a carboxyl group at position 126 and a positive charge at position 144 are absolutely required.