Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens
- A. Glaser, N. Reder, Jonathan T. C. Liu
- MedicineNature Biomedical Engineering
- 3 June 2017
It is shown that an open-top light-sheet microscope optimized for non-destructive slide-free pathology of clinical specimens enables the rapid imaging of intact tissues at high resolution over large 2D and 3D fields of view, with the same level of detail as traditional pathology.
Cherenkov radiation fluence estimates in tissue for molecular imaging and therapy applications
- A. Glaser, Rongxiao Zhang, J. Andreozzi, D. Gladstone, B. Pogue
- PhysicsSPIE BiOS
- 13 August 2015
Quantitative estimates of the light fluence rate of Cherenkov radiation from both radionuclides and radiotherapy beams in a biological tissue are presented for the first time and indicate that diagnostic potential is reasonable for Cherennikov excitation of molecular probes, but phototherapy may remain elusive at such exceedingly low fluence values.
Optical dosimetry of radiotherapy beams using Cherenkov radiation: the relationship between light emission and dose
- A. Glaser, Rongxiao Zhang, D. Gladstone, B. Pogue
- Physics, MedicinePhysics in Medicine and Biology
- 21 July 2014
For x-ray photons the light emission would be optimally suited for narrow beam stereotactic radiation therapy and surgery validation studies, for verification of dynamic intensity-modulated and volumetric modulated arc therapy treatment plans in water tanks, near monoenergetic sources and also for entrance and exit surface imaging dosimetry of both narrow and broad beams.
Microscopy with ultraviolet surface excitation for wide-area pathology of breast surgical margins
- Weisi Xie, Ye Chen, Jonathan T. C. Liu
- MedicineJournal of Biomedical Optics
- 1 February 2019
The image quality of the MUSE system is compared with both frozen-section and conventional H&E histology, demonstrating the feasibility to provide microscopic visualization of breast margin surfaces at speeds that are relevant for intraoperative use.
Harnessing non-destructive 3D pathology
- Jonathan T. C. Liu, A. Glaser, A. Madabhushi
- BiologyNature Biomedical Engineering
- 15 February 2021
An overview of the imaging technologies that enable 3D pathology, and the associated computational tools—machine learning, in particular—for image processing and interpretation are provided.
Raman-Encoded Molecular Imaging with Topically Applied SERS Nanoparticles for Intraoperative Guidance of Lumpectomy.
- Y. Wang, N. Reder, Jonathan T. C. Liu
- Medicine, BiologyCancer Research
- 15 August 2017
A Raman-encoded molecular imaging technique in which targeted nanoparticles are topically applied on excised tissues to enable rapid visualization of a multiplexed panel of cell surface biomarkers at surgical margin surfaces highlights the sensitivity and specificity of REMI to detect biomarkers in freshly resected tissue, which has the potential to reduce the rate of re-excision procedures in cancer patients.
Projection imaging of photon beams using ÄŒerenkov-excited fluorescence
- A. Glaser, S. Davis, W. Voigt, Rongxiao Zhang, B. Pogue, D. Gladstone
- Physics, MedicinePhysics in Medicine and Biology
- 7 February 2013
A novel optical-based dose imaging approach using a standard commercial camera, water tank, and fluorescent dye, which when excited by the ÄŒerenkov emission induced by the radiation beam, allows 2D projection imaging in a fast timeframe, potentially leading toward 3D tomographic beam profiling.
A GAMOS plug-in for GEANT4 based Monte Carlo simulation of radiation-induced light transport in biological media
- A. Glaser, S. Kanick, Rongxiao Zhang, P. Arce, B. Pogue
- PhysicsBiomedical Optics Express
- 17 April 2013
A tissue optics plug-in that interfaces with the GEANT4/GAMOS Monte Carlo (MC) architecture is described, providing a means of simulating radiation-induced light transport in biological media for the first time and showing excellent agreement with currently employed biomedical optics MC codes.
Three-dimensional ÄŒerenkov tomography of energy deposition from ionizing radiation beams.
- A. Glaser, W. Voigt, S. Davis, Rongxiao Zhang, D. Gladstone, B. Pogue
- PhysicsOptics Letters
- 1 March 2013
A novel application of the ÄŒerenkov effect for the recovery of the spatial distribution of ionizing radiation energy deposition in a medium is explored and the successful three-dimensional tomographic reconstruction of the imparted dose distribution is demonstrated.
Superficial dosimetry imaging of ÄŒerenkov emission in electron beam radiotherapy of phantoms
- Rongxiao Zhang, C. Fox, A. Glaser, D. Gladstone, B. Pogue
- Physics, MedicinePhysics in Medicine and Biology
- 21 August 2013
Measurements and simulations indicate that ÄŒerenkov emission imaging might provide a valuable method of superficial dosimetry imaging from incident radiotherapy beams of electrons.
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