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Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs
MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic… Expand
MicroRNAs Regulate Brain Morphogenesis in Zebrafish
MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally. To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZdicer) mutants that disrupt… Expand
A Novel miRNA Processing Pathway Independent of Dicer Requires Argonaute2 Catalytic Activity
No Dicer for Me MicroRNAs (miRNAs) are small noncoding RNAs found in most eukaryotes. Most are processed from primary transcripts in the nucleus by the microprocessor enzyme complex, which includes… Expand
Identification of small ORFs in vertebrates using ribosome footprinting and evolutionary conservation
Identification of the coding elements in the genome is a fundamental step to understanding the building blocks of living systems. Short peptides (< 100 aa) have emerged as important regulators of… Expand
Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition
After fertilization, maternal factors direct development and trigger zygotic genome activation (ZGA) at the maternal-to-zygotic transition (MZT). In zebrafish, ZGA is required for gastrulation and… Expand
HSPG modification by the secreted enzyme Notum shapes the Wingless morphogen gradient.
The secreted signaling protein Wingless acts as a morphogen to pattern the imaginal discs of Drosophila. Here we report identification of a secreted repressor of Wingless activity, which we call… Expand
CRISPRscan: designing highly efficient sgRNAs for CRISPR/Cas9 targeting in vivo
- M. Moreno-Mateos, Charles E. Vejnar, +4 authors A. Giraldez
- Biology, Medicine
- Nature Methods
- 30 July 2015
CRISPR-Cas9 technology provides a powerful system for genome engineering. However, variable activity across different single guide RNAs (sgRNAs) remains a significant limitation. We analyzed the… Expand
Ribosome Profiling Shows That miR-430 Reduces Translation Before Causing mRNA Decay in Zebrafish
Translation Block MicroRNAs (miRNAs) are small, noncoding RNA genes that are found in the genomes of most eukaryotes, where they play an important role in the regulation of gene expression. Although… Expand
Opposing activities of Dally-like glypican at high and low levels of Wingless morphogen activity.
The glypican family of heparan sulfate proteoglycans has been implicated in formation of morphogen gradients. Here, we examine the role of the glypican Dally-like protein (Dlp) in shaping the… Expand
Zygotic genome activation during the maternal-to-zygotic transition.
- M. T. Lee, Ashley R. Bonneau, A. Giraldez
- Medicine, Biology
- Annual review of cell and developmental biology
- 6 October 2014
Embryogenesis depends on a highly coordinated cascade of genetically encoded events. In animals, maternal factors contributed by the egg cytoplasm initially control development, whereas the zygotic… Expand