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QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects
SUMMARY QDD is an open access program providing a user-friendly tool for microsatellite detection and primer design from large sets of DNA sequences. Expand
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Underestimation of Species Richness in Neotropical Frogs Revealed by mtDNA Analyses
Background Amphibians are rapidly vanishing. At the same time, it is most likely that the number of amphibian species is highly underestimated. Recent DNA barcoding work has attempted to define aExpand
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Accuracy and quality assessment of 454 GS-FLX Titanium pyrosequencing
BackgroundThe rapid evolution of 454 GS-FLX sequencing technology has not been accompanied by a reassessment of the quality and accuracy of the sequences obtained. Current strategies forExpand
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QDD version 3.1: a user‐friendly computer program for microsatellite selection and primer design revisited: experimental validation of variables determining genotyping success rate
Microsatellite marker development has been greatly simplified by the use of high‐throughput sequencing followed by in silico microsatellite detection and primer design. However, the selection ofExpand
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High‐throughput microsatellite isolation through 454 GS‐FLX Titanium pyrosequencing of enriched DNA libraries
Microsatellites (or SSRs: simple sequence repeats) are among the most frequently used DNA markers in many areas of research. The use of microsatellite markers is limited by the difficulties involvedExpand
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Revealing cryptic diversity using molecular phylogenetics and phylogeography in frogs of the Scinax ruber and Rhinella margaritifera species groups.
Few studies to date have examined genetic variability of widespread tropical amphibian species over their distributional range using different kinds of molecular markers. Here, we use genetic data inExpand
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Evidence of en bloc duplication in vertebrate genomes
It has been 30 years since it was first proposed that the vertebrate genome evolved through several rounds of genome-wide duplications (polyploidizations). Despite rapid advances in genetics,Expand
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Insight into the origin of endemic Mediterranean ichthyofauna: phylogeography of Chondrostoma genus (Teleostei, Cyprinidae).
The Chondrostoma genus is widespread in Europe, with numerous endemic species in northern Mediterranean rivers. We reconstructed the phylogenetic relationships of this genus, using the wholeExpand
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Phylogeography of the vairone (Leuciscus souffia, Risso 1826) in Central Europe
The vairone Leuciscus souffia is a cyprinid fish that inhabits river systems in and around the Alps. The complete mitochondrial DNA control region (945 bp) was sequenced in 295 vairone from 22Expand
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A co-evolution perspective of the TNFSF and TNFRSF families in the immune system.
Within the 'success story' of vertebrate evolution, the acquisition and refinement of the adaptive immune system is far from the least impressive example of co-evolution. Members of the tumourExpand
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