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A unique method to produce transgenic embryos in ovine, porcine, feline, bovine and equine species.
Transgenesis is an essential tool in many biotechnological applications. Intracytoplasmic sperm injection (ICSI)-mediated gene transfer is a powerful technique to obtain transgenic pups; however,Expand
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Procedure for maximizing oocyte harvest for in vitro embryo production in small ruminants.
Possible effects of repeated hormonal treatments and laparoscopic ovum pick-up (LOPU) on the efficiency of oocyte recovery rate and quality were determined in sheep and goats. In six adult MerinoExpand
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A simple vitrification technique for sheep and goat embryo cryopreservation
Abstract The aim of this study was to evaluate pregnancy and embryo survival rate of vitrified in vivo produced Merino sheep and Criolla goat (morulae and blastocysts) embryos, using the plastic tipsExpand
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Timing of emergence of ovulatory follicles in polyovulatory goats.
The current study characterized the timing of emergence of ovulatory follicles during the follicular phase of the estrous cycle in polyovulatory does and assessed whether selection may influenceExpand
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Repeatability of superovulatory response to successive FSH treatments in Merino sheep
Abstract The efficiency of a multiple ovulation and embryo transfer program depends on the recovery of a large number of viable embryos per donor ewe at each successive superovulatory treatment.Expand
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Technical recommendations for artificial insemination in sheep
Abstract Artificial insemination (AI) was the first important biotechnology applied to improve the genetics of farm animals. It allows the rapid and massive diffusion of desirable characteristics ofExpand
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Vitrified embryo transfer in Merino sheep under extensive conditions
Abstract The aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips in Merino sheep under extensive conditions. A second objective was to evaluate theExpand
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Cryopreservation and diffusion of goat genetic material in the Argentine Patagonia
The high demand from goat Argentinian breeders for improving their Angora herds determined the need of implementing a genetic breeding program based on the use of artificial insemination (AI) withExpand
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Efficiency of sperm-mediated gene transfer in the ovine by laparoscopic insemination, in vitro fertilization and ICSI.
Transgenesis constitutes an important tool for pharmacological protein production and livestock improvement. We evaluated the potential of laparoscopic insemination (LI), in vitro fertilization (IVF)Expand
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