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PELDOR in rotationally symmetric homo-oligomers
Nanometre distance measurements by pulsed electron−electron double resonance (PELDOR) spectroscopy have become an increasingly important tool in structural biology. The theoretical underpinning ofExpand
Sub‐Micromolar Pulse Dipolar EPR Spectroscopy Reveals Increasing CuII‐labelling of Double‐Histidine Motifs with Lower Temperature
Abstract Electron paramagnetic resonance (EPR) distance measurements are making increasingly important contributions to the studies of biomolecules by providing highly accurate geometric constraints.Expand
DEER distance measurements on trityl/trityl and Gd(iii)/trityl labelled proteins.
Triarylmethyl (TAM or trityl) radicals are becoming important for measuring distances in proteins and nucleic acids. Here, we report on a new trityl spin label CT02MA, which conjugates to a proteinExpand
Assessing dimerisation degree and cooperativity in a biomimetic small-molecule model by pulsed EPR.
Pulsed electron paramagnetic resonance (EPR) spectroscopy is gaining increasing importance as a complementary biophysical technique in structural biology. Here, we describe the synthesis,Expand
Nitroxide-nitroxide and nitroxide-metal distance measurements in transition metal complexes with two or three paramagnetic centres give access to thermodynamic and kinetic stabilities.
Fundamentally, the stability of coordination complexes and of templated (bio)macromolecular assemblies depends on the thermodynamic and kinetic properties of the intermediates and final complexesExpand
Monitoring Complex Formation by Relaxation‐Induced Pulse Electron Paramagnetic Resonance Distance Measurements
Abstract Biomolecular complexes are often multimers fueling the demand for methods that allow unraveling their composition and geometric arrangement. Pulse electron paramagnetic resonance (EPR)Expand
Orientation selection in high-field RIDME and PELDOR experiments involving low-spin CoII ions.
Orientation selective (OS) RIDME and PELDOR were conducted on a low-spin CoII complex coordinated by two nitroxide (NO) labelled 2,2':6',2''-terpyridine ligands. Co-NO RIDME at W- and Q-band gaveExpand
Two closed ATP- and ADP-dependent conformations in yeast Hsp90 chaperone detected by Mn(II) EPR spectroscopic techniques
Significance Hsp90 is a homo-dimeric chaperone regulating clients involved in tumorigenesis and signal transduction. Its mechanism of action depends on ATP hydrolysis, which is coupled to aExpand
Small neutral Gd(iii) tags for distance measurements in proteins by double electron-electron resonance experiments.
Spin labels containing a Gd(iii) ion have become important for measuring nanometer distances in proteins by double electron-electron resonance (DEER) experiments at high EPR frequencies. The distanceExpand
Cell‐Free Synthesis of Selenoproteins in High Yield and Purity for Selective Protein Tagging
The selenol group of selenocysteine is much more nucleophilic than the thiol group of cysteine. Selenocysteine residues in proteins thus offer reactive points for rapid post‐translationalExpand