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Role of steroid sulfatase in steroid homeostasis and characterization of the sulfated steroid pathway: Evidence from steroid sulfatase deficiency
Expression of human CYP27A1 in B. megaterium for the efficient hydroxylation of cholesterol, vitamin D3 and 7-dehydrocholesterol.
2β- and 16β-hydroxylase activity of CYP11A1 and direct stimulatory effect of estrogens on pregnenolone formation
Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium
- A. Gerber, Michael Kleser, R. Biedendieck, R. Bernhardt, F. Hannemann
- BiologyMicrobial Cell Factories
- 29 July 2015
A whole-cell system based on B. megaterium enables the conversion of the steroid hormone precursor cholesterol to pregnenolone in substantial quantities and demonstrates that the microorganism’s PHB granules, aggregates of bioplastic coated with a protein/phospholipid monolayer, are crucial for the high conversion rate by serving as substrate storage.
A Novel NADPH-dependent flavoprotein reductase from Bacillus megaterium acts as an efficient cytochrome P450 reductase.
Functionalized poly(3-hydroxybutyric acid) bodies as new in vitro biocatalysts.
A novel short chain dehydrogenase from Bacillus megaterium for the conversion of the sesquiterpene nootkatol to (+)-nootkatone.
Genetic engineering of Bacillus megaterium for high-yield production of the major teleost progestogens 17α,20β-di- and 17α,20β,21α-trihydroxy-4-pregnen-3-one.
Human CYP27A1 catalyzes hydroxylation of β-sitosterol and ergosterol
- Maximilian Ehrhardt, A. Gerber, J. Zapp, F. Hannemann, R. Bernhardt
- Biology, ChemistryBiological chemistry
- 1 June 2016
Abstract β-Sitosterol and ergosterol are the equivalents of cholesterol in plants and fungi, respectively, and common sterols in the human diet. In the current work, both were identified as novel…
Products of gut-microbial tryptophan metabolism inhibit the steroid hormone-synthesizing cytochrome P450 11A1
An inhibitory effect of indole and skatole on CYP11A1 is described, leading to a decrease in pregnenolone formation, the precursor of all mineralocorticoids, glucocortICoids, and sex steroids.