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Immobilisation of Antibodies and Antigens on Macro Solid Phases — A Comparison Between Adsorptive and Covalent Binding. A critical study of macro solid phases for use in immunoassay systems, Part I
TLDR
A comparison between adsorption and covalent binding of protein-conjugates, antibodies, antibodies and haptens to polystyrene, nylon and glass balls is described.
Synthesis and Evaluation of Luminescent Tracers and Hapten-Protein Conjugates for Use in Luminescence Immunoassays with Immobilised Antibodies and Antigens. A critical study of macro solid phases for
This article describes the synthesis of labels and hapten-protein conjugates for use in bio- and chemiluminescent immunoassay systems, together with the problems encountered. The effects of maleimide
An Evaluation of Four Different Luminescence Immunoassay Systems: CELIA (chemiluminescent immunoassay), SPALT (solid-phase antigen luminescence technique), ILMA (immunoluminometric assay) and ILSA
TLDR
Luminescence immunoassays with coefficients of variation comparable with radioimmunoassay have been designed, values of under 5% being obtainable within the working range of the assay.
An Evaluation of Four Different Luminescence Immunoassay Systems: CELIA (chemiluminescent immunoassay), SPALT (solid-phase antigen luminescence technique), ILMA (immunoluminometric assay), and ILSA
The perfonnance of different solid-phase luminescence immunoassays has been documented using four different assay concepts. These are CELIA (chemiluminescence immunoassay), SPALT (solid-phase antigen
Gramicidin S synthetase. Stability of reactive thioester intermediates and formation of 3-amino-2-piperidone.
TLDR
The reactive thioester complexes of gramicidin S synthetase with substrate amino acids and intermediate peptides are slowly hydrolyzed in neutral buffer solutions under mild conditions and a different decomposition pattern is observed for the thioesters complex of GS2 with LOrn.
Gramicidin S synthetase. Temperature dependence and thermodynamic parameters of substrate amino acid activation reactions.
In the biosynthesis of the cyclic decapeptide antibiotic gramicidin S, the constituent amino acids are activated by a two-step mechanism involving aminoacyl adenylate and thio ester formation which
Formation of D-Phe-Pro-Val-cyclo-Orn by gramicidin S synthetase in the absence of L-leucine.
TLDR
D-Phe-Pro-Val-cyclo-Orn was obtained as a product of the multienzyme by omission of L-leucine from the complete bioassay mixture and was identified and characterized by chromatographic and spectroscopic procedures using chemically synthesized reference compounds.
Streptoquin and Septazole: Antibiotic Drugs as CorrosionInhibitors for Copper in Aqueous Solutions
The inhibiting effect of streptoquin and septazole towards the corrosion of the copper in 0.1M of HCl solution has been studied by potentiodynamic polarization measurements, electrochemical impedance
Patents and literature
Bioassays, including immunoassays, enzyme assays, and assays using enzyme electrodes, and nucleic acid hybridization probes have been the subject of considerable industrial and academic research. New
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