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The current state of two‐dimensional electrophoresis with immobilized pH gradients
The original protocol of two‐dimensional electrophoresis with immobilized pH gradient (IPG‐Dalt; Görg et al., Electrophoresis 1988, 9, 531—546) is updated. Merits and limits of different methods forExpand
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Current two-dimensional electrophoresis technology for proteomics.
Two-dimensional gel electrophoresis (2-DE) with immobilized pH gradients (IPGs) combined with protein identification by mass spectrometry (MS) is currently the workhorse for proteomics. In spite ofExpand
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Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis.
In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver stainingExpand
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Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications.
A new technique for generating pH gradients in isoelectric focusing is described, based on the principle that the buffering groups are covalently linked to the matrix used as anticonvective medium.Expand
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Very alkaline immobilized pH gradients for two‐dimensional electrophoresis of ribosomal and nuclear proteins
Basic proteins normally lost by the cathodic drift of carrier ampholyte focusing, or separated by NEPHGE with limited reproducibility, could be well separated by two‐dimensional (2‐D) electrophoresisExpand
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2‐DE with IPGs
In order to overcome the limitations of carrier ampholyte generated pH gradients, IPGs were developed in the late 1970s. However, the 2‐DE pattern we included in the first publication on IEF withExpand
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Elimination of point streaking on silver stained two‐dimensional gels by addition of iodoacetamide to the equilibration buffer
Silver staining artifacts, unrelated to protein, such as horizontal lines and vertical micro or point streaking on two‐dimensional gels with carrier ampholytes or immobilized pH gradients in theExpand
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Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants
Glyoxysomes are a subclass of peroxisomes involved in lipid mobilization. Two distinct peroxisomal targeting signals (PTSs), the C-terminal PTS1 and the N-terminal PTS2, are defined. Processing ofExpand
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Recent developments in two‐dimensional gel electrophoresis with immobilized pH gradients: Wide pH gradients up to pH 12, longer separation distances and simplified procedures
Wide‐range immobilized pH 3—12 and 6—12 gradients were generated. Depending on the extraction method of sample preparation, proteins with pIs up to pH 11.7 were resolved. Highly reproducible proteinExpand
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Two-dimensional electrophoresis
Recent developments in the first- and second-dimension steps of two-dimensional electrophoresis have improved pattern reproducibility.
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