A. Fulton

Publications19
h-index6
Citations154
Highly Influential Citations2
Claim Author Page
Author pages are created from data sourced from our academic publisher partnerships and public sources.

Recommended Authors

  • H. Gohlke
  • 215 Publications, 13,289 Citations
  • K. Jaeger
  • 120 Publications, 3,898 Citations
  • U. Schwaneberg
  • 182 Publications, 2,789 Citations
  • J. Büchs
  • 478 Publications, 9,337 Citations
  • Publications
  • Influence
Unraveling the effects of amino acid substitutions enhancing lipase resistance to an ionic liquid: a molecular dynamics study.
Understanding of the structural and dynamic properties of enzymes in non-aqueous media (e.g., ionic liquids, ILs) is highly attractive for protein engineers and synthetic biochemists. Despite a… Expand
  • 5
  • 1
  • PDF
Systematically Scrutinizing the Impact of Substitution Sites on Thermostability and Detergent Tolerance for Bacillus subtilis Lipase A
TLDR
We extend our knowledge on guidelines for protein engineering following a data-driven approach by scrutinizing the impact of substitution sites on thermostability or / and detergent tolerance for Bacillus subtilis lipase A (BsLipA) at very large scale. Expand
  • 3
  • 1
  • PDF
Exploring the Protein Stability Landscape: Bacillus subtilis Lipase A as a Model for Detergent Tolerance
A systematic study was conducted with Bacillus subtilis lipase A (BSLA) to determine the effect of every single amino acid substitution on detergent tolerance. BSLA is a minimal α/β‐hydrolase of 181… Expand
  • 31
Application of Rigidity Theory to the Thermostabilization of Lipase A from Bacillus subtilis
TLDR
We describe and validate a novel, ensemble-based, yet highly efficient strategy based on the CNA approach to predict optimal amino acid substitutions at structural weak spots for improving a protein’s thermostability. Expand
  • 26
  • PDF
Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli
BackgroundEscherichia coli is commonly used in academia and industry for expressing recombinant proteins because of its well-characterized molecular genetics and the availability of numerous… Expand
  • 39
Towards Understanding Directed Evolution: More than Half of All Amino Acid Positions Contribute to Ionic Liquid Resistance of Bacillus subtilis Lipase A
Ionic liquids (ILs) are attractive (co‐)solvents for biocatalysis. However, in high concentration (>10 % IL), enzymes usually show decreased activity. No general principles have been discovered to… Expand
  • 25
Optimal Scanning of All Single-Point Mutants of a Protein
TLDR
We model and analyze an iterative two-stage procedure to generate all single-point mutants of a protein, and derive its optimal value given the experimental parameters. Expand
  • 8
  • PDF
A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase
Allostery, i.e. the control of enzyme activity by a small molecule at a location distant from the enzyme’s active site, represents a mechanism essential for sustaining life. The rational design of… Expand
  • 6
Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance
ObjectivesProtein engineering has been employed to successfully improve organic solvent resistance of enzymes. Exploration of nature’s full potential (how many beneficial positions/beneficial… Expand
  • 8
High-Throughput Screening Assays for Lipolytic Enzymes.
Screening is defined as the identification of hits within a large library of variants of an enzyme or protein with a predefined property. In theory, each variant present in the respective library… Expand
  • 1