Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
- A. Fire, Siqun Xu, M. Montgomery, S. Kostas, Samuel E. Driver, C. Mello
- 19 February 1998
To their surprise, it was found that double-stranded RNA was substantially more effective at producing interference than was either strand individually, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.
Genes and Mechanisms Related to RNA Interference Regulate Expression of the Small Temporal RNAs that Control C. elegans Developmental Timing
Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans.
Specific interference by ingested dsRNA
This work shows that C. elegans can respond in a gene-specific manner to dsRNA encountered in the environment, and finds that Escherichia coli bacteria expressing dsRNAs can confer specific interference effects on the nematode larvae that feed on them.
On the Role of RNA Amplification in dsRNA-Triggered Gene Silencing
The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegans
A modular set of lacZ fusion vectors for studying gene expression in Caenorhabditis elegans.
Loss of the Putative RNA-Directed RNA Polymerase RRF-3 Makes C. elegans Hypersensitive to RNAi
Distinct Populations of Primary and Secondary Effectors During RNAi in C. elegans
Analysis of endogenous small RNAs indicated that a fraction derive from a biosynthetic mechanism that is similar to that of secondary siRNAs formed during RNAi, suggesting that small antisense transcripts derived from cellular messenger RNAs by RdRP activity may have key roles in cellular regulation.
A high-resolution, nucleosome position map of C. elegans reveals a lack of universal sequence-dictated positioning.
These analyses provide a global view of the chromatin architecture of a multicellular animal at extremely high density and resolution and release this data set, via the UCSC Genome Browser, as a resource for the high-resolution analysis of chromatin conformation and DNA accessibility at individual loci within the C. elegans genome.