• Publications
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Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities.
PU.1 is a member of the ets family of transcription factors and is expressed exclusively in cells of the hematopoietic lineage. Mice homozygous for a disruption in the PU.1 DNA binding domain areExpand
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CCCTC-binding factor (CTCF) and cohesin influence the genomic architecture of the Igh locus and antisense transcription in pro-B cells
Compaction and looping of the ~2.5-Mb Igh locus during V(D)J rearrangement is essential to allow all VH genes to be brought in proximity with DH-JH segments to create a diverse antibody repertoire,Expand
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Lack of N regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences
  • A. Feeney
  • Biology, Medicine
  • The Journal of experimental medicine
  • 1 November 1990
Much of T and B lymphocyte receptor diversity derives from the addition of nontemplated N regions at the junctions of receptor gene elements, although fetal T cells expressing gamma/delta receptorsExpand
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Both E12 and E47 allow commitment to the B cell lineage.
The E2A gene products, E12 and E47, are required for proper B cell development. Mice lacking the E2A gene products generate only a very small number of B220+ cells, which lack immunoglobulin DJ(H)Expand
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Noncoding transcription within the Igh distal VH region at PAIR elements affects the 3D structure of the Igh locus in pro-B cells
Noncoding sense and antisense germ-line transcription within the Ig heavy chain locus precedes V(D)J recombination and has been proposed to be associated with Igh locus accessibility, although itsExpand
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Tcra gene recombination is supported by a Tcra enhancer- and CTCF-dependent chromatin hub
Antigen receptor locus V(D)J recombination requires interactions between widely separated variable (V), diversity (D), and joining (J) gene segments, but the mechanisms that generate theseExpand
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Deep Sequencing of the Murine Igh Repertoire Reveals Complex Regulation of Nonrandom V Gene Rearrangement Frequencies
A diverse Ab repertoire is formed through the rearrangement of V, D, and J segments at the IgH (Igh) loci. The C57BL/6 murine Igh locus has >100 functional VH gene segments that can recombine to aExpand
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Individual V(H) promoters vary in strength, but the frequency of rearrangement of those V(H) genes does not correlate with promoter strength nor enhancer-independence.
The process of V(D)J recombination is highly regulated. Germline transcription of unrearranged gene segments precedes V(D)J rearrangement, and the correlation between germline transcription andExpand
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Unifying model for molecular determinants of the preselection Vβ repertoire
Significance The assembly of immunoglobulin and T-cell receptor genes by V(D)J (variable, diversity, joining) recombination must strike a balance between maximum diversification of antigen receptorsExpand
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Cutting Edge: Developmental Stage-Specific Recruitment of Cohesin to CTCF Sites throughout Immunoglobulin Loci during B Lymphocyte Development1
Contraction of the large Igh and Igκ loci brings all V genes, spanning >2.5 Mb in each locus, in proximity to DJH or Jκ genes. CCCTC-binding factor (CTCF) is a transcription factor that regulatesExpand
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