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Matrix Elasticity Directs Stem Cell Lineage Specification
Microenvironments appear important in stem cell lineage specification but can be difficult to adequately characterize or control with soft tissues. Naive mesenchymal stem cells (MSCs) are shown hereExpand
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Substrate compliance versus ligand density in cell on gel responses.
Substrate stiffness is emerging as an important physical factor in the response of many cell types. In agreement with findings on other anchorage-dependent cell lineages, aortic smooth muscle cellsExpand
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Preparation of Hydrogel Substrates with Tunable Mechanical Properties
  • J. R. Tse, A. Engler
  • Materials Science, Medicine
  • Current protocols in cell biology
  • 1 June 2010
The modulus of elasticity of the extracellular matrix (ECM), often referred to in a biological context as “stiffness,” naturally varies within the body, e.g., hard bones and soft tissue. Moreover, itExpand
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Myotubes differentiate optimally on substrates with tissue-like stiffness
Contractile myocytes provide a test of the hypothesis that cells sense their mechanical as well as molecular microenvironment, altering expression, organization, and/or morphology accordingly. Here,Expand
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Embryonic cardiomyocytes beat best on a matrix with heart-like elasticity: scar-like rigidity inhibits beating
Fibrotic rigidification following a myocardial infarct is known to impair cardiac output, and it is also known that cardiomyocytes on rigid culture substrates show a progressive loss of rhythmicExpand
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Stem cell fate dictated solely by altered nanotube dimension
Two important goals in stem cell research are to control the cell proliferation without differentiation and to direct the differentiation into a specific cell lineage when desired. Here, weExpand
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Matrix stiffness drives Epithelial-Mesenchymal Transition and tumour metastasis through a TWIST1-G3BP2 mechanotransduction pathway
Matrix stiffness potently regulates cellular behaviour in various biological contexts. In breast tumours, the presence of dense clusters of collagen fibrils indicates increased matrix stiffness andExpand
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Interplay of Matrix Stiffness and Protein Tethering in Stem Cell Differentiation
Stem cells regulate their fate by binding to, and contracting against, the extracellular matrix. Recently, it has been proposed that in addition to matrix stiffness and ligand type, the degree ofExpand
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Intrinsic extracellular matrix properties regulate stem cell differentiation.
One of the recent paradigm shifts in stem cell biology has been the discovery that stem cells can begin to differentiate into mature tissue cells when exposed to intrinsic properties of theExpand
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Power-law rheology of isolated nuclei with deformation mapping of nuclear substructures.
Force-induced changes in genome expression as well as remodeling of nuclear architecture in development and disease motivate a deeper understanding of nuclear mechanics. Chromatin and greenExpand
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