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Retroviral intasome assembly and inhibition of DNA strand transfer
Integrase is an essential retroviral enzyme that binds both termini of linear viral DNA and inserts them into a host cell chromosome. The structure of full-length retroviral integrase, eitherExpand
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Flexible use of nuclear import pathways by HIV-1.
HIV-1 replication requires transport of nascent viral DNA and associated virion proteins, the retroviral preintegration complex (PIC), into the nucleus. Too large for passive diffusion throughExpand
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Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases.
HIV integrase is the enzyme responsible for inserting the viral DNA into the host chromosome; it is essential for HIV replication. The crystal structure of the catalytically active core domainExpand
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Identification of Host Proteins Required for HIV Infection Through a Functional Genomic Screen
HIV-1 exploits multiple host proteins during infection. We performed a large-scale small interfering RNA screen to identify host factors required by HIV-1 and identified more than 250 HIV-dependencyExpand
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Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75.
Integrase (IN) is an essential retroviral enzyme, and human transcriptional coactivator p75, which is also referred to as lens epithelium-derived growth factor (LEDGF), is the dominant cellularExpand
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LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration.
LEDGF/p75 directly interacts with lentiviral integrase proteins and can modulate their enzymatic activities and chromosomal association. A novel genetic knockout model was established that allowed usExpand
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Integrase-mediated spacer acquisition during CRISPR–Cas adaptive immunity
Bacteria and archaea insert spacer sequences acquired from foreign DNAs into CRISPR loci to generate immunological memory. The Escherichia coli Cas1–Cas2 complex mediates spacer acquisition in vivo,Expand
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The Requirement for Cellular Transportin 3 (TNPO3 or TRN-SR2) during Infection Maps to Human Immunodeficiency Virus Type 1 Capsid and Not Integrase
ABSTRACT Recent genome-wide screens have highlighted an important role for transportin 3 in human immunodeficiency virus type 1 (HIV-1) infection and preintegration complex (PIC) nuclear import.Expand
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HIV-1 DNA integration: Mechanism of viral DNA cleavage and DNA strand transfer
Retroviral DNA integration involves a coordinated set of DNA cutting and joining reactions. Linear viral DNA is cleaved at each 3' end to generate the precursor ends for integration. The resultingExpand
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Multiple effects of mutations in human immunodeficiency virus type 1 integrase on viral replication.
The integration of a DNA copy of the human immunodeficiency virus type 1 (HIV-1) genome into a chromosome of an infected cell is a pivotal step in virus replication. Integration requires the activityExpand
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