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Polycystins 1 and 2 mediate mechanosensation in the primary cilium of kidney cells
PC1 and PC2 contribute to fluid-flow sensation by the primary cilium in renal epithelium and that they both function in the same mechanotransduction pathway, suggesting loss or dysfunction of PC1 or PC2 may lead to polycystic kidney disease. Expand
Proteomic Screen Finds pSer/pThr-Binding Domain Localizing Plk1 to Mitotic Substrates
This finding reveals how Plk1 can localize to specific sites within cells in response to Cdk phosphorylation at those sites and provides a structural mechanism for targeting the PlK1 kinase domain to its substrates. Expand
The Molecular Basis for Phosphodependent Substrate Targeting and Regulation of Plks by the Polo-Box Domain
Mutations that specifically disrupt phosphodependent interactions abolish cell-cycle-dependent localization and provide compelling phenotypic evidence that PBD-phospholigand binding is necessary for proper mitotic progression. Expand
MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation.
It is proposed that MAPKAP kinase-2 is a new member of the DNA damage checkpoint kinase family that functions in parallel with Chk1 and Chk2 to integrate DNA damage signaling responses and cell cycle arrest in mammalian cells. Expand
The CD225 Domain of IFITM3 Is Required for both IFITM Protein Association and Inhibition of Influenza A Virus and Dengue Virus Replication
It is demonstrated that similar molecular determinants are needed for IFITM3's restriction of both IAV and DENV, however, a portion of the CIL including Y99 and R87 is preferentially needed for inhibition of the orthomyxovirus. Expand
RFWD3-Dependent Ubiquitination of RPA Regulates Repair at Stalled Replication Forks.
It is demonstrated that RPA, which functions as a protein scaffold in the replication stress response, is multiply ubiquitinated upon replication fork stalling, and mutations suggest that multisite ubiquitination of the entire RPA complex is responsible for repair at stalled forks. Expand
Quantitative Proteomic Atlas of Ubiquitination and Acetylation in the DNA Damage Response.
This work finds that K6- and K33-linked polyubiquitination undergo bulk increases in response to DNA damage, raising the possibility that these linkages are largely dedicated to DDR function, and shows that Cullin-RING ligases mediate 10% of DNA damage-induced ubiquitination events. Expand
Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening
It is determined by peptide library selection and phosphosite array screening that the protein kinase Dbf2-Mob1 preferentially phosphorylated substrates that contain an RXXS motif, and a subsequent proteome microarray screen revealed proteins that can be phosphorylation by Dbf1 in vitro. Expand
Decreased UDP‐GlcNAc levels abrogate proliferation control in EMeg32‐deficient cells
EMeg32‐dependent UDP‐GlcNAc levels influence cell cycle progression and susceptibility to apoptotic stimuli and, Interestingly, growth‐impaired EMeg32−/− MEFs withstand a number of apoptotic stimulus and express activated PKB/AKT. Expand
Chromatin proteins captured by ChIP–mass spectrometry are linked to dosage compensation in Drosophila
X-chromosome dosage compensation by the MSL (male-specific lethal) complex is required in Drosophila melanogaster to increase gene expression from the single male X to equal that of both female XExpand