New insights on trehalose: a multifunctional molecule.
- A. Elbein, Y. T. Pan, I. Pastuszak, D. Carroll
- BiologyGlycobiology
- 1 April 2003
This sugar is present in a wide variety of organisms, including bacteria, yeast, fungi, insects, invertebrates, and lower and higher plants, where it may serve as a source of energy and carbon and as a signaling molecule to direct or control certain metabolic pathways or even to affect growth.
Inhibitors of the biosynthesis and processing of N-linked oligosaccharide chains.
- A. Elbein
- Biology, ChemistryAnnual Review of Biochemistry
- 1987
A number of glycoproteins have oligosaccharides linked to protein in a GlcNAc----asparagine bond that are either of the complex, the high-mannose or the hybrid structure and a number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport.
Kifunensine, a potent inhibitor of the glycoprotein processing mannosidase I.
- A. Elbein, J. Tropea, M. Mitchell, G. Kaushal
- Biology, ChemistryJournal of Biological Chemistry
- 15 September 1990
Kifunensine appears to be one of the most effective glycoprotein processing inhibitors observed thus far, and knowledge of its structure may lead to the development of potent inhibitors for other processing enzymes.
Inhibitors of the biosynthesis and processing of N-linked oligosaccharides.
- A. Elbein
- Biology, ChemistryCRC Critical Reviews in Biochemistry
- 1984
A number of glycoproteins have oligosaccharides linked to protein in a GlcNAc----asparagine bond that are either of the complex, the high-mannose or the hybrid structure and a number of inhibitors have been identified that interfere with glycoprotein biosynthesis, processing, or transport.
Trehalose synthase of Mycobacterium smegmatis: purification, cloning, expression, and properties of the enzyme.
- Yuan T. Pan, Vineetha Koroth Edavana, A. Elbein
- BiologyEuropean Journal of Biochemistry
- 1 November 2004
The catalytic mechanism may involve scission of the incoming disaccharide and transfer of a glucose to an enzyme-bound glucose, as [(3)H]glucose incubated with TreS and either unlabeled maltose or trehalose results in formation of [(3]H]disaccharides, suggesting that this enzyme may be valuable in carbohydrate synthetic chemistry.
Mechanism of action of tunicamycin on the UDP-GlcNAc:dolichyl-phosphate Glc-NAc-1-phosphate transferase.
- A. Heifetz, R. Keenan, A. Elbein
- ChemistryBiochemistry
- 29 May 1979
Last Step in the Conversion of Trehalose to Glycogen
- A. Elbein, I. Pastuszak, A. Tackett, T. Wilson, Yuan T. Pan
- Biology, ChemistryJournal of Biological Chemistry
- 29 January 2010
It is proposed that trehalose synthase, maltokinase, and GMPMT represent a new pathway of glycogen synthesis using tre Halose as the source of glucose.
Purification, Cloning, Expression, and Properties of Mycobacterial Trehalose-phosphate Phosphatase*
- Stacey Klutts, I. Pastuszak, A. Elbein
- BiologyJournal of Biological Chemistry
- 24 January 2003
The trehalose-phosphate phosphatase (TPP) was purified from the cytosol of Mycobacterium smegmatis to near homogeneity using a variety of conventional steps to achieve a purification of about…
Glycosidase inhibitors: inhibitors of N‐linked oligosaccharide processing
- A. Elbein
- Biology, ChemistryThe FASEB Journal
- 1 December 1991
A number of alkaloid‐like compounds have been identified that are specific inhibitors of the glucosidases and mannosidases involved in glycoprotein processing, causing the formation of glycoproteins with glucose‐containing high mannose structures, or various high‐mannose or hybrid chains, depending on the site of inhibition.
A novel trehalase from Mycobacterium smegmatis − purification, properties, requirements
- J. D. Carroll, I. Pastuszak, V. Edavana, Yuan T. Pan, A. Elbein
- BiologyThe FEBS Journal
- 1 April 2007
The purified trehalase showed a single 71 kDa band on SDS gels, but active enzyme eluted in the void volume of a Sephracryl S‐300 column, suggesting a molecular mass of about 1500‰kDa or a multimer of 20 or more subunits.
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