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Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes
Results indicate that in vivo DNA superhelicity is a result of a balance between topoisomerase I and gyrase activities, and an excess of negative supercoils due to an absence of topoisomersase I is deleterious to the cell, but a moderate gyrases deficiency is not harmful. Expand
A yeast origin of replication is activated late in S phase
The finding that 67 kb of telomere-adjacent DNA at the right end of chromosome V in S. cerevisiae is replicated late in S phase supports the idea that the pattern of interspersed early and late replication along the chromosomes of higher eukaryotes is a consequence of the temporal regulation of origin activation. Expand
Enhancement of bacterial gene expression by insertion elements or by mutation in a CAP-cAMP binding site.
The ethylmethane sulfonate-induced point mutations in bglR are alterations in a single base in the cAMP binding protein (CAP) binding site, leading to a stronger binding of the CAP-cAMP complex. Expand
Insertion of DNA activates the cryptic bgl operon in E. coli K12
Two insertion elements, IS1 and IS5, have been observed to effect activation of the cryptic bgl operon of Escherichia coli K12 and the operon is inducible by βglucosides in a cyclic AMP-dependent manner. Expand
Roles of the 2 microns gene products in stable maintenance of the 2 microns plasmid of Saccharomyces cerevisiae.
Two different assays show that the level of Flp activity decreases with increasing 2 microns copy number, and the overexpression of FLP in a strain carrying endogenous 2 micron leads to uncontrolled plasmid replication, longer cell cycles, and cell death. Expand
Positive and negative regulation of the bgl operon in Escherichia coli
It is proposed that the bglS gene product has an additional role as a component of the beta-glucoside transport system. Expand
Time of replication of ARS elements along yeast chromosome III.
The results suggest that some ARS elements in the chromosome may be inactive as replication origins, and chromosome III sequences were found to replicate late in G1 at the arrest induced by the temperature-sensitive cdc7 allele. Expand
Vital and mortal staining of algae in the presence of chlorine-produced oxidants
High concentrations of CPO affected staining with neutral red in several instances; this is probably due to CPO-induced mortality of test organisms during the exposure or staining period. Expand
Roles of the 2 , um Gene Products in Stable Maintenance of the 2 iim Plasmid of Saccharomyces cerevisiae
We have examined the replication and segregation of the Saccharomyces cerevisiae 2,um circle. The amplification of the plasmid at low copy numbers requires site-specific recombination between theExpand
Growth and Manipulation of Yeast
This unit describes preparation of selected media for growing yeast and also discusses strain storage and revival. Protocols are provided for the assay of β‐galactosidase in liquid culture and forExpand