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Extended screening by PCR for seven cry-group genes from field-collected strains of Bacillus thuringiensis.
An extended multiplex PCR method was established to rapidly identify and classify Bacillus thuringiensis strains containing cry (crystal protein) genes toxic to species of Lepidoptera, Coleoptera,Expand
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Stripping of digoxigenin-labeled probes from nylon membranes.
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Chemiluminescent detection of DNA on nylon membranes.
Two types of nonradioactive DNA detection systems were optimized for use with nylon membranes in Southern transfers. A luminol substrate system (consisting of an enhanced chemiluminescent reactionExpand
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Sensitive fluorescent detection of protein on nylon membranes.
Detection of antigen immobilized on membranes, as in Western transfers and dot enzyme linked immunosorbent assays (ELISAs), often employ antibody-enzyme conjugates and chemiluminescent orExpand
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Monoclonal antibodies and immobilized antibodies
Antibodies in both their free and immobilized state have been the object of considerable industrial and academic interest. A variety of methods are used for preparing and immobilizing antibodies.Expand
Tutorial : Comparing Different Membranes and Fixation Methods
T he cellular response to experimental or environmental stimulus can be more readily understood when subpopulations of RNA are harvested and characterized. This gives the researcher an insight intoExpand