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Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid, and the sequenced genome revealed a large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors. Expand
Protein translocation across the bacterial cytoplasmic membrane.
This review summarizes the present knowledge of the mechanism and structure of the Sec translocase, with a special emphasis on unresolved questions and topics of current research. Expand
Sec- and Tat-mediated protein secretion across the bacterial cytoplasmic membrane--distinct translocases and mechanisms.
In bacteria, two major pathways exist to secrete proteins across the cytoplasmic membrane: the general Secretion route and the Twin-arginine translocation pathway, which catalyses the translocation of secretory proteins in their folded state. Expand
Δμ H+ and ATP function at different steps of the catalytic cycle of preprotein translocase
Preprotein translocation in E. coli requires ATP, the membrane electrochemical potential delta mu H+, and translocase, an enzyme with an ATPase domain (SecA) and the membrane-embedded SecY/E. StudiesExpand
The purified E. coli integral membrane protein SecY E is sufficient for reconstitution of SecA-dependent precursor protein translocation
The isolation of the SecY/E protein, the integral membrane protein component of the E. coli preprotein translocase, is reported, which consists of SecY, SecE, and an additional polypeptide. Expand
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase
It is proposed that YidC, homologous to Saccharomyces cerevisiae Oxa1p, is involved in the insertion of hydrophobic sequences into the lipid bilayer after initial recognition by the SecAYEG translocase. Expand
A presequence- and voltage-sensitive channel of the mitochondrial preprotein translocase formed by Tim23
Proteins imported into the mitochondrial matrix are synthesized in the cytosol with an N-terminal presequence and are translocated through hetero-oligomeric translocase complexes of the outer and inner mitochondrial membranes, where purified Tim23 forms a hydrophilic, ∼13–24 Å wide channel characteristic of the mitochondrial presequence translocases. Expand
The molecular chaperone SecB is released from the carboxy‐terminus of SecA during initiation of precursor protein translocation
It is concluded that the SecB‐binding site on SecA is confined to the extreme carboxy‐terminus of the SecA dimer, and that SecB is released from this site at the onset of translocation. Expand
Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum
Genes predicted to encode transporters were strongly overrepresented among the genes transcriptionally upregulated under conditions that stimulate penicillinG production, illustrating potential for future genomics-driven metabolic engineering. Expand
Identification of Diverse Archaeal Proteins with Class III Signal Peptides Cleaved by Distinct Archaeal Prepilin Peptidases
A diverse set ofarchaeal proteins with class III signal peptides that might be subunits of as-yet-undescribed cell surface structures, such as archaeal pili are revealed. Expand