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Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabaseExpand
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Protein translocation across the bacterial cytoplasmic membrane.
About 25% to 30% of the bacterial proteins function in the cell envelope or outside of the cell. These proteins are synthesized in the cytosol, and the vast majority is recognized as a ribosome-boundExpand
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Sec- and Tat-mediated protein secretion across the bacterial cytoplasmic membrane--distinct translocases and mechanisms.
In bacteria, two major pathways exist to secrete proteins across the cytoplasmic membrane. The general Secretion route, termed Sec-pathway, catalyzes the transmembrane translocation of proteins inExpand
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ΔμH+ and ATP function at different steps of the catalytic cycle of preprotein translocase
Preprotein translocation in E. coli requires ATP, the membrane electrochemical potential delta mu H+, and translocase, an enzyme with an ATPase domain (SecA) and the membrane-embedded SecY/E. StudiesExpand
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The molecular chaperone SecB is released from the carboxy‐terminus of SecA during initiation of precursor protein translocation
The chaperone SecB keeps precursor proteins in a translocation‐competent state and targets them to SecA at the translocation sites in the cytoplasmic membrane of Escherichia coli. SecA is thought toExpand
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Identification of diverse archaeal proteins with class III signal peptides cleaved by distinct archaeal prepilin peptidases.
Most secreted archaeal proteins are targeted to the membrane via a tripartite signal composed of a charged N terminus and a hydrophobic domain, followed by a signal peptidase-processing site. SignalExpand
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Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum
Industrial penicillin production with the filamentous fungus Penicillium chrysogenum is based on an unprecedented effort in microbial strain improvement. To gain more insight into penicillinExpand
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YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase
In Escherichia coli, both secretory and inner membrane proteins initially are targeted to the core SecYEG inner membrane translocase. Previous work has also identified the peripherally associatedExpand
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A presequence- and voltage-sensitive channel of the mitochondrial preprotein translocase formed by Tim23
Proteins imported into the mitochondrial matrix are synthesized in the cytosol with an N-terminal presequence and are translocated through hetero-oligomeric translocase complexes of the outer andExpand
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Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1.
Resistance of Lactococcus lactis to cytotoxic compounds shares features with the multidrug resistance phenotype of mammalian tumor cells. Here, we report the gene cloning and functionalExpand
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