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Crystal Structure of the Nuclear Export Receptor CRM1 in Complex with Snurportin1 and RanGTP
The structure of an exportin complex shows how nuclear transport complexes differentially recognize cargo and suggests that RanGTP promotes cargo-binding to CRM1 solely through long-range conformational changes in the exportin. Expand
A Role for RanBP1 in the Release of CRM1 from the Nuclear Pore Complex in a Terminal Step of Nuclear Export
The results indicate that RanGTP is important for the targeting of export complexes to the cytoplasmic side of the NPC and that RanBP1 and probably RanBP2 are involved in the dissociation of nuclear export complexes from the NPC in a terminal step of transport. Expand
Conserved Stem II of the Box C/D Motif Is Essential for Nucleolar Localization and Is Required, Along with the 15.5K Protein, for the Hierarchical Assembly of the Box C/D snoRNP
It is revealed that the binding of 15.5K to the box C/D motif is essential for the association of the remaining snoRNP-associated proteins, namely, NOP56, Nop58, fibrillarin, and the nucleoplasmic proteins TIP48 and TIP49. Expand
Nucleocytoplasmic Shuttling Factors Including Ran and CRM1 Mediate Nuclear Export of NFAT In Vitro
These studies directly demonstrate by fractionation and reconstitution that nuclear export of NFAT is mediated by multiple nucleocytoplasmic shuttling factors, including Ran and CRM1. Expand
Molecular Basis for Multiple Sulfatase Deficiency and Mechanism for Formylglycine Generation of the Human Formylglycine-Generating Enzyme
It is reported that FGE is a single-domain monomer with a surprising paucity of secondary structure and adopts a unique fold, providing a molecular basis of multiple sulfatase deficiency (MSD), a fatal autosomal recessive syndrome. Expand
Crystal structure of the polysialic acid–degrading endosialidase of bacteriophage K1F
The crystal structures of endosialidase NF and its complex with oligomeric sialic acid indicate that the unique polySia specificity evolved from a combination of structural elements characteristic of exosilidases and bacteriophage tailspike proteins. Expand
The Velvet Family of Fungal Regulators Contains a DNA-Binding Domain Structurally Similar to NF-κB
This study reveals an important family of fungal regulatory proteins to be transcription factors that contain a DNA-binding “velvet” domain structurally related to that of mammalian NFkB.
Crystal structure of a bacterial class 2 histone deacetylase homologue.
The structure of the bacterial FB188 HDAH (histone deacetylase-like amidohydrolase from Bordetella/Alcaligenes strain FB188) is presented that reveals high sequential and functional homology to human class 2 HDACs and exhibits the canonical fold of class 1HDACs. Expand
Structural basis for m3G‐cap‐mediated nuclear import of spliceosomal UsnRNPs by snurportin1
The crystal structure of the m3G‐cap‐binding domain of snurportin1 with bound m3gpppG at 2.4 Å resolution is reported, revealing a structural similarity to the mRNA‐guanyly‐transferase. Expand
A jack of all trades: the multiple roles of the unique essential second messenger cyclic di‐AMP
The components of the c‐di‐AMP signaling system are reviewed to review the molecular mechanisms behind this molecule, which is implicated in a variety of functions in the cell, including cell wall metabolism, potassium homeostasis, DNA repair and the control of gene expression. Expand