Methanotrophs and copper.
- J. Semrau, A. DiSpirito, Sukhwan Yoon
- Biology, Environmental ScienceFEMS Microbiology Reviews
- 1 July 2010
The current state of knowledge of the phylogeny, environmental distribution, and potential applications of methanotrophs for regional and global issues are summarized, as well as the role of Cu in regulating gene expression and proteome in these cells, its effects on enzymatic and whole-cell activity, and the novel Cu uptake system used by methanOTrophs are summarized.
Membrane-associated methane monooxygenase from Methylococcus capsulatus (Bath)
- J. Zahn, A. DiSpirito
- Biology, ChemistryJournal of Bacteriology
- 1 February 1996
An active preparation of the membrane-associated methane monooxygenase (pMMO) from Methylococcus capsulatus Bath was isolated by ion-exchange and hydrophobic interaction chromatography using dodecyl…
Characterization of volatile organic emissions and wastes from a swine production facility
- J. Zahn, J. Hatfield, Y. Do, A. DiSpirito, D. Laird, R. Pfeiffer
- Environmental Science
- 1 November 1997
Analytical methods for monitoring the volatile organic compound (VOC) emissions, for defining atmospheric transport coefficients, and for monitoring solution phase chemistry have been developed to…
Methanobactin, a Copper-Acquisition Compound from Methane-Oxidizing Bacteria
- H. J. Kim, D. Graham, P. Sherwood
- 10 September 2004
Evidence of analogous molecules for copper transport from methane-oxidizing bacteria is presented, represented by a small fluorescent chromopeptide produced by Methylosinus trichosporium OB3b.
Functional classification of swine manure management systems based on effluent and gas emission characteristics.
- J. Zahn, J. Hatfield, D. Laird, T. T. Hart, Y. Do, A. DiSpirito
- Environmental Science, MedicineJournal of Environmental Quality
- 1 March 2001
A classification system based on gas emission characteristics and effluent concentrations of total phosphorus (P) and total sulfur (S) was devised and tested on 29 swine manure management systems in Iowa, Oklahoma, and North Carolina in an effort to elucidate functional characteristics of these systems.
The Membrane-Associated Methane Monooxygenase (pMMO) and pMMO-NADH:Quinone Oxidoreductase Complex from Methylococcus capsulatus Bath
- D. Choi, Ryan C. Kunz, A. DiSpirito
- BiologyJournal of Bacteriology
- 1 October 2003
It is demonstrated that copper not only regulates the metabolic switch between the two methane monooxygenases but also regulates the level of expression of the pMMO and the development of internal membranes.
Spectral, kinetic, and thermodynamic properties of Cu(I) and Cu(II) binding by methanobactin from Methylosinus trichosporium OB3b.
- D. Choi, Corbin J. Zea, A. DiSpirito
- 10 January 2006
To examine the potential role of methanobactin (mb) as the extracellular component of a copper acquisition system in Methylosinus trichosporium OB3b, the metal binding properties of mb were examined and suggested copper coordination changes at different Cu(II):mb ratios.
NMR, mass spectrometry and chemical evidence reveal a different chemical structure for methanobactin that contains oxazolone rings.
- L. Behling, S. Hartsel, W. Gallagher
- Chemistry, BiologyJournal of the American Chemical Society
- 27 August 2008
NMR, mass spectrometry, and chemical data reveal a chemical structure for methanobactin that is significantly different than the previously published one and indicates that oxazolone rings instead of hydroxyimidazolate rings form the core of the two chromophoric residues.
Correlation of human olfactory responses to airborne concentrations of malodorous volatile organic compounds emitted from swine effluent.
- J. Zahn, A. DiSpirito, Y. Do, B. E. Brooks, E. E. Cooper, J. Hatfield
- Environmental Science, BiologyJournal of Environmental Quality
- 1 March 2001
Results of this study show that direct multicomponent analysis of VOCs emitted from swine effluent can be applied toward estimating perceived odor intensity.
Copper-Binding Compounds from Methylosinus trichosporium OB3b
- A. DiSpirito, J. Zahn, A. Taylor
- ChemistryJournal of Bacteriology
- 15 July 1998
Two copper-binding compounds/cofactors were isolated from the spent media of both the wild type and a constitutive soluble methane monooxygenase mutant, PP319, and high concentrations of CBCs were observed in the extracellular fraction of the sMMOC mutants PP319 and PP359 regardless of the copper concentration in the culture medium.