N-linked glycosylation in Campylobacter jejuni and its functional transfer into E. coli.
It is demonstrated that a functional N-linked glycosylation pathway could be transferred into Escherichia coli and opened up the possibility of engineering permutations of recombinant glycan structures for research and industrial applications.
GlycoWorkbench: a tool for the computer-assisted annotation of mass spectra of glycans.
- A. Ceroni, K. Maass, H. Geyer, R. Geyer, A. Dell, S. Haslam
- BiologyJournal of Proteome Research
- 1 March 2008
GlycoWorkbench is a software tool developed by the EUROCarbDB initiative to assist the manual interpretation of MS data to evaluate a set of structures proposed by the user by matching the corresponding theoretical list of fragment masses against the list of peaks derived from the spectrum.
Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli.
- M. Feldman, M. Wacker, M. Aebi
- Biology, EngineeringProceedings of the National Academy of Sciences…
- 22 February 2005
E. coli cells are engineered in a way that two different pathways, protein N-glycosylation and lipopolysaccharide (LPS) biosynthesis, converge at the step in which PglB, the key enzyme of the C. jejuni N-gresylation system, transfers O polysaccharides from a lipid carrier (undecaprenyl pyrophosphate) to an acceptor protein.
Symbol Nomenclature for Graphical Representations of Glycans.
- A. Varki, R. Cummings, S. Kornfeld
- Philosophy, MedicineGlycobiology
- 1 December 2015
Author(s): Varki, Ajit; Cummings, Richard D; Aebi, Markus; Packer, Nicole H; Seeberger, Peter H; Esko, Jeffrey D; Stanley, Pamela; Hart, Gerald; Darvill, Alan; Kinoshita, Taroh; Prestegard, James J;…
Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies.
- E. Yates, J. Valdor, J. P. Knox
- BiologyGlycobiology
- 1 March 1996
Interestingly, the most effective inhibitor of the binding of the monoclonal antibodies MAC207, JIM4 and JIM13 to exudate gum antigens was an acidic trisaccharide, isolated from a partial acid hydrolysate of gum karaya which has the structure: GlcA beta(1-->3) GalA alpha(1)--Rha, determined by a combination of FAB-MS, GC-MS and NMR spectroscopy.
Molecular characterization of the surface layer proteins from Clostridium difficile
- Emanuela Calabi, S. Ward, N. Fairweather
- BiologyMolecular Microbiology
- 1 June 2001
To the authors' knowledge, this is the first example in which two SLPs have been shown to derive from a single gene product through post‐translational processing, rather than from the expression of separate genes.
Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome.
- Cory D. Rillahan, A. Antonopoulos, J. Paulson
- Biology, ChemistryNature Chemical Biology
- 1 July 2012
It is shown that fluorinated analogs of sialic acid and fucose can be taken up and metabolized to the desired donor substrate-based inhibitors inside the cell, resulting in a global, family-wide shutdown of sIALyl- and/or fucosyltransferases and remodeling of cell-surface glycans.
Glycolipids as Receptors for Bacillus thuringiensis Crystal Toxin
- J. Griffitts, S. Haslam, R. Aroian
- Biology, ChemistryScience
- 11 February 2005
It is demonstrated that the major mechanism for Bt toxin resistance in Caenorhabditis elegans entails a loss of glycolipid carbohydrates, and evidence that insect Glycolipids are also receptors for BT toxin is presented, showing carbohydrate-dependent and relevant for toxin action in vivo.
The detection, purification, structural characterization, and metabolism of diphosphoinositol pentakisphosphate(s) and bisdiphosphoinositol tetrakisphosphate(s).
- L. Stephens, T. Radenberg, G. Mayr
- Biology, ChemistryJournal of Biological Chemistry
- 25 February 1993
Identification of xanthurenic acid as the putative inducer of malaria development in the mosquito
- O. Billker, V. Lindo, H. Morris
- BiologyNature
- 19 March 1998
It is shown that low concentrations of xanthurenic acid can act together with pH to induce gametogenesis in vitro, and could form the basis of the rational development of new methods of interrupting the transmission of malaria using drugs or new refractory mosquito genotypes to block parasitegametogenesis.
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