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Biodegradation of phenol at high initial concentrations in two-phase partitioning batch and fed-batch bioreactors.
A two-phase organic-aqueous system was used to degrade phenol in both batch and fed-batch culture. The solvent, which contained the phenol and partitioned it into the aqueous phase, was… Expand
Two-phase partitioning bioreactors: a new technology platform for destroying xenobiotics.
- A. Daugulis
- Biology, Medicine
- Trends in biotechnology
- 1 November 2001
Toxic organic compounds (xenobiotics) pose serious environmental and health risks worldwide. Biological treatment of these materials is severely constrained by their toxic and inhibitory nature and… Expand
Solvent Selection Strategies for Extractive Biocatalysis
This report follows the development of systematic solvent screening strategies for the identification of superior pure solvents and introduces techniques for the identification of effective… Expand
A rational approach to improving productivity in recombinant Pichia pastoris fermentation.
A Mut(S) Pichia pastoris strain that had been genetically modified to produce and secrete sea raven antifreeze protein was used as a model system to demonstrate the implementation of a rational,… Expand
Mixed-feed exponential feeding for fed-batch culture of recombinant methylotrophic yeast
A simple, accurate model capable of predicting cell growth and methanol utilization during the mixed substrate fed-batch fermentation of MutS recombinant Pichia pastoris was developed and was used to… Expand
Bioproduction of the aroma compound 2‐Phenylethanol in a solid–liquid two‐phase partitioning bioreactor system by Kluyveromyces marxianus
The rose‐like aroma compound 2‐phenylethanol (2‐PE) is an important fragrance and flavor ingredient. Several yeast strains are able to convert l‐phenylalanine (l‐phe) to 2‐PE among which… Expand
Interfacial effects in a two-phase partitioning bioreactor: degradation of polycyclic aromatic hydrocarbons (PAHs) by a hydrophobic Mycobacterium
The growth of Mycobacterium PYR-1 on polycyclic aromatic hydrocarbons (PAHs) was examined in a two-phase partitioning bioreactor (TPPB). TPPBs are characterized by a cell-containing aqueous phase,… Expand
Alginate‐Polylysine Microcapsules of Controlled Membrane Molecular Weight Cutoff for Mammalian Cell Culture Engineering
The search for more productive mammalian cell culture techniques has been primarily driven by the need to increase both the cell density and product concentration. In the technique used in our… Expand
Enhanced biodegradation of phenol by a microbial consortium in a solid–liquid two phase partitioning bioreactor
Two phase partitioning bioreactors (TPPBs) operate by partitioning toxic substrates to or from an aqueous, cell-containing phase by means of second immiscible phase. Uptake of toxic substrates by the… Expand
Degradation of xenobiotics in a partitioning bioreactor in which the partitioning phase is a polymer
- B. Amsden, Justyna Bochanysz, A. Daugulis
- Chemistry, Medicine
- Biotechnology and bioengineering
- 20 November 2003
Two‐phase partitioning bioreactors (TPPBs) are characterized by a cell‐containing aqueous phase and a second immiscible phase that contains toxic and/or hydrophobic substrates that partition to the… Expand