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Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances.
Microalgae represent an exceptionally diverse but highly specialized group of micro-organisms adapted to various ecological habitats. Many microalgae have the ability to produce substantial amountsExpand
HaloTag: a novel protein labeling technology for cell imaging and protein analysis.
We have designed a modular protein tagging system that allows different functionalities to be linked onto a single genetic fusion, either in solution, in living cells, or in chemically fixed cells.Expand
Genetic Engineering of Algae for Enhanced Biofuel Production
ABSTRACT There are currently intensive global research efforts aimed at increasing and modifying the accumulation of lipids, alcohols, hydrocarbons, polysaccharides, and other energy storageExpand
The promise and challenges of microalgal‐derived biofuels
Microalgae offer great promise to contribute a significant portion of the renewable fuels that will be required by the Renewable Fuels Standard described in the 2007 Energy Independence and SecurityExpand
Theoretical Maximum Algal Oil Production
Interest in algae as a feedstock for biofuel production has risen in recent years, due to projections that algae can produce lipids (oil) at a rate significantly higher than agriculture-basedExpand
Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium
  • A. Darzins
  • Biology, Medicine
  • Molecular microbiology
  • 1 January 1994
The type 4 pill of Pseudomonas aeruginosa are important cell‐associated virulence factors that play a crucial role in mediating (i) bacterial adherence to, and colonization of, mucosal surfaces, (ii)Expand
The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY.
  • A. Darzins
  • Biology, Medicine
  • Journal of bacteriology
  • 1 September 1993
The Pseudomonas aeruginosa pilG gene, encoding a protein which is involved in pilus production, was cloned by phenotypic complementation of a unique, pilus-defective mutant of strain PAO1. ThisExpand
DNA shuffling method for generating highly recombined genes and evolved enzymes
We introduce a method of in vitro recombination or “DNA shuffling” to generate libraries of evolved enzymes. The approach relies on the ordering, trimming, and joining of randomly cleaved parentalExpand
Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa
Virulence of the opportunistic pathogen Pseudomonas aeruginosa involves the coordinate expression of a wide range of virulence factors including type IV pili which are required for colonization ofExpand
Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga
Biofuels derived from algal lipids represent an opportunity to dramatically impact the global energy demand for transportation fuels. Systems biology analyses of oleaginous algae could greatlyExpand
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