• Publications
  • Influence
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances.
TLDR
A brief summary of the current knowledge on oleaginous algae and their fatty acid and TAG biosynthesis, algal model systems and genomic approaches to a better understanding of TAG production, and a historical perspective and path forward for microalgae-based biofuel research and commercialization are provided.
HaloTag: a novel protein labeling technology for cell imaging and protein analysis.
TLDR
The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.
Genetic Engineering of Algae for Enhanced Biofuel Production
TLDR
Potential avenues of genetic engineering that may be undertaken in order to improve microalgae as a biofuel platform for the production of biohydrogen, starch-derived alcohols, diesel fuel surrogates, and/or alkanes are focused on.
The promise and challenges of microalgal‐derived biofuels
Microalgae offer great promise to contribute a significant portion of the renewable fuels that will be required by the Renewable Fuels Standard described in the 2007 Energy Independence and Security
Theoretical Maximum Algal Oil Production
Interest in algae as a feedstock for biofuel production has risen in recent years, due to projections that algae can produce lipids (oil) at a rate significantly higher than agriculture-based
Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium
  • A. Darzins
  • Biology
    Molecular microbiology
  • 1 January 1994
The type 4 pill of Pseudomonas aeruginosa are important cell‐associated virulence factors that play a crucial role in mediating (i) bacterial adherence to, and colonization of, mucosal surfaces, (ii)
The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY
  • A. Darzins
  • Biology
    Journal of bacteriology
  • 1 September 1993
TLDR
Results suggest that PilG may be an important part of the signal transduction system involved in the elaboration of P. aeruginosa pili.
Cloning of genes controlling alginate biosynthesis from a mucoid cystic fibrosis isolate of Pseudomonas aeruginosa
TLDR
Mucoid strains of Pseudomonas aeruginosa isolated from the sputum of cystic fibrosis patients produce copious quantities of an exopolysaccharide known as alginic acid, which has been difficult to isolate individual structural gene mutants defective in alginate synthesis.
DNA shuffling method for generating highly recombined genes and evolved enzymes
TLDR
This method represents a conceptually distinct and improved alternative to sexual PCR for gene family shuffling and is demonstrated by molecular breeding of a monooxygenase for increased rate and extent of biodesulfurization on complex substrates, as well as for 20-fold faster conversion of a nonnatural substrate.
Examination of Triacylglycerol Biosynthetic Pathways via De Novo Transcriptomic and Proteomic Analyses in an Unsequenced Microalga
TLDR
The results indicate an upregulation of both fatty acid and triacylglycerol biosynthetic machinery under oil-accumulating conditions, and demonstrate the utility of a de novo assembled transcriptome as a search model for proteomic analysis of an unsequenced microalga.
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